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Full-Text Articles in Engineering

Near Miss Reporting: Perspectives On Worker Conversance Of Incident Events Across Two Industries, Julia Mcgee Jan 2019

Near Miss Reporting: Perspectives On Worker Conversance Of Incident Events Across Two Industries, Julia Mcgee

University of the Pacific Theses and Dissertations

The practice of reporting workplace incident events is adopted as best practice by organizations and complies with Occupational Safety and Health Administration (OSHA) mandates. Reporting the near miss incident type in which no injury or damage to equipment or the environment occurs is buttressed by the assumption that both the worker and the organization ascribe to the same goal to identify workplace hazards and prevent incident recurrence. The goal of incident reporting is not apparent, and the path to achieving the goal is obstructed by internal and external hazards that act to oppose the reporting process, such that the goals …


Expression, Purification, And Characterization Of Recombinant Basic Fibroblast Growth Factor In Pichia Pastoris, Henry Hieu Minh Le Jan 2019

Expression, Purification, And Characterization Of Recombinant Basic Fibroblast Growth Factor In Pichia Pastoris, Henry Hieu Minh Le

University of the Pacific Theses and Dissertations

Wounds in the mouth, occurring after oral surgery, take time to heal. No ointment can be added to help with the healing process because mouth saliva will constantly wash it away. In order to combat this problem, we propose engineering a normal flora microbe to grow at the site of injury and secrete a recombinant growth factor to promote healing of the damaged tissue. Our goal is to have the yeast Pichia pastoris produce human basic fibroblast growth factor (bFGF), which aids in cellular proliferation. P. pastoris is a good choice for this application because not only is it considered …