Engineering Commons^™

Peptide Nanotube Encapsulated Enzyme Biosensor For Vapor Phase Detection Of Malathion, An Organophosphorus Compound, Christopher W. Edwards, Surachet Duanghathaipornsuk, Mark N. Goltz, Sushil Kanel, Dong-Shik Kim

Faculty Publications

This study explores the use of a butyrylcholinesterase (BChE)-based, reversible reaction biosensor using screen-printed electrodes (SPEs) having a smaller working surface area than the single-use electrodes previously studied. Previous research demonstrated the prospective application of a single-use biosensor fabricated with an acetylcholinesterase (AChE) enzyme encapsulated in peptide nanotubes (PNTs) and enhanced with horseradish peroxidase (HRP) to detect organophosphorus compounds (OPCs) in aqueous and gas phases. In the current study, potential improvements to the biosensor are investigated. BChE-based biosensors were fabricated using PNTs, HRP, and Nafion in combination to increase the reactive surface area, enhance sensitivity, and maintain enzyme stability. Cyclic …