Engineering Commons^™

Production Of A Nanoplasmid™ With A Large Gene Insert Using The Hypergro™ Fermentation Process, Aaron Carnes, Neha Tiwari, Jill Beilowitz, Carlos Sampson, Dorothy Peterson, Jim Williams

Vaccine Technology VI

Plasmid based DNA vaccines are emerging as a promising alternative to traditional vaccines due to several advantages, including faster production of DNA plasmids using E. coli. However, further increases in transgene expression are needed to meet efficacy requirements for various non-viral gene therapy and DNA vaccination applications. While existing minicircle DNA technology has been shown to offer improved levels and durations of transgene expression by removal of the bacterial region from the plasmid, low manufacturing yields may be a barrier to widespread use of minicircle DNA for vaccination.

NTC’s minimalized Nanoplasmid™ vectors utilize RNA-OUT (R_OUT) antibiotic-free selection …