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A New Approximate Model For Microscopy Imaging, Heidy Sierra, Dana Brooks, Charles Dimarzio

Dana Brooks

There has been increasing interest in recent years in techniques for microscopic examination of optically thick transparent objects. A number of phase imaging modalities have been developed to address this need. If a stack of images is acquired through focusing, the image at a given focal plane is contaminated by out-of-focus information coming from other planes [1]. There is a need to develop 3D imaging models for phase microscopes that will allow deconvolution, or more generally inverse reconstruction, techniques to be developed. Thus there is a need for an image formation model for phase microscopy that is able to maintain …