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Full-Text Articles in Engineering
Development Of Plant Cell Culture Processes To Produce Natural Product Pharmaceuticals: Characterization, Analysis, And Modeling Of Plant Cell Aggregation, Martin Kolewe
Open Access Dissertations
Plant derived natural products represent some of the most effective anti-cancer and anti-infectious disease pharmaceuticals available today. However, uncertainty regarding the feasibility of commercial supply due to the limited availability of many plants in nature has resulted in a dramatic reduction in the use of natural products as leads in modern drug discovery. Plant cell suspension culture, consisting of dedifferentiated plant cells grown in vitro and amenable to large scale industrial biotechnology processes, is a production alternative which promises renewable and economical supply of these important drugs. The widespread application of this technology is limited by low product yields, slow …
Analysis Of Aggregate Size As A Process Variable Affecting Paclitaxel Accumulation In Taxus Suspension Cultures, Martin E. Kolewe, Michael A. Henson, Susan C. Roberts
Analysis Of Aggregate Size As A Process Variable Affecting Paclitaxel Accumulation In Taxus Suspension Cultures, Martin E. Kolewe, Michael A. Henson, Susan C. Roberts
Michael A Henson
Plant cell aggregates have long been implicated in affecting cellular metabolism in suspension culture, yet the rigorous characterization of aggregate size as a process variable and its effect on bioprocess performance has not been demonstrated. Aggregate fractionation and analysis of biomass-associated product is commonly used to assess the effect of aggregation, but we establish that this method is flawed under certain conditions and does not necessarily agree with comprehensive studies of total culture performance. Leveraging recent advances to routinely measure aggregate size distributions, we developed a simple method to manipulate aggregate size and evaluate its effect on the culture as …