Engineering Commons^™

Utilization Of Rice Bran By Pythium Irregulare For Lipid Production, Hui Zhu

LSU Master's Theses

This research investigated the feasibility of utilizing rice bran as feedstock for submerged fermentation of Pythium irregulare to produce fungal lipids, which contain polyunsaturated fatty acids (PUFAs) including arachidonic acid (AA, C20:4n6) and eicosapentaenoic acid (EPA, C20:5n3). The fungal nutrient requirements, temperature characterization and growth modeling were determined in glucose-yeast extract media. Sixteen media combinations based on a 24 factorial design with four levels of glucose and yeast extract ranged from 1% to 4% and 0.25% to 1%, respectively, and corresponding carbon/nitrogen ratio (C/N) ranged from 4 to 64, were examined. The suitable C/N for Pythium irregulare growth ranged from …