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Full-Text Articles in Engineering
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Cells and Materials
A comparative study of the behavior of chick embryo endothelial cells grown on various substrates was performed in order to establish the reliability and the limitation of both cell and organ culture methods. Following substrates were analyzed to compare these two different culture techniques: bovine serum albumin, pigskin gelatin and albumin + gelatin cross-linked by glutaraldehyde or carbodiimide, fibrin glue and negative control (Thermanox*). Parameters of cell growth and adhesion were calculated and compared with electron microscopic observations of cell morphology and of the extracellular matrix. Both culture methods provided complementary results and led to a similar classification of the …
Changes In Intracellular Ca2+ And Structure In Platelets Contacting Synthetic Substrates, L. M. Waples, O. E. Olorundare, S. L. Goodman, R. M. Albrecht
Changes In Intracellular Ca2+ And Structure In Platelets Contacting Synthetic Substrates, L. M. Waples, O. E. Olorundare, S. L. Goodman, R. M. Albrecht
Cells and Materials
Platelet structural changes such as spreading and cytoskeletal reorganization that occur upon contact with synthetic surfaces have been well documented . Structural changes such as spreading will vary depending on the substrate involved . Although platelet structural changes following adhesion have been recorded , the cellular mechanisms including changes in intracellular calcium flux underlying these platelet responses are less well understood. In this study, video microscopy was used to image platelet adhesion and spreading while simultaneously imaging intracellular free Ca2+ levels in individual Fura-2 loaded human platelets. This was accomplished using fluorescence video microscopy coupled with video enhanced asymmetric illumination …
Reactogenicity Of Biomaterials As Studied By Biochemical, Morphological And Ultrastructural Techniques, Leonid I. Slutskii, Natalya A. Sevastjanova, Ivetta L. Ozolanta, Irina V. Kuzmina, Laimdota E. Dombrovska
Reactogenicity Of Biomaterials As Studied By Biochemical, Morphological And Ultrastructural Techniques, Leonid I. Slutskii, Natalya A. Sevastjanova, Ivetta L. Ozolanta, Irina V. Kuzmina, Laimdota E. Dombrovska
Cells and Materials
Reactogenicity is a characteristic of biocompatible materials that provokes the reparative and proliferative reaction of connective tissues, a compulsory stage of which is inflammation. Thus, reactogenicity studies should include experiments in vivo. A quantitative assessment of reactogenicity can be obtained by subcutaneous implantation of standard olive-shaped specimens covered by the biomaterial under study followed by biochemical, histological and scanning electron microscopical studies of the capsule developing around the implant. Reactogenicity of surgical threads is evaluated by semiquantitative histological analysis of the wound healing process after suture application. Biomaterial reactogenicity can be modified by changing the structure of the surface and …
Review: Biomaterials For Abdominal Wall Reconstruction, Roger K. J. Simmermacher, Robert P. Bleichrodt, Jeff M. Schakenraad
Review: Biomaterials For Abdominal Wall Reconstruction, Roger K. J. Simmermacher, Robert P. Bleichrodt, Jeff M. Schakenraad
Cells and Materials
The reconstruction of large abdominal wall defects still is a major surgical problem. Many different techniques have been developed for this purpose, most of which appeared to be unsatisfactory. The lack of sufficient tissue requires the insertion of prosthetic material. Non-absorbable prostheses used to reconstruct abdominal wall defects showed the best results . Polypropylene mesh (PPM) and expanded polytetrafluoroethylene (ePTFE) soft-tissue patch are the most frequently used materials for this purpose . However, PPM induces extensive visceral adhesions and erosion of the skin, whereas ePTFE is insufficiently anchored to the adjacent tissue and therefore both materials are not ideal. As …