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Biomedical Engineering and Bioengineering
Weldon School of Biomedical Engineering Faculty Working Papers
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- Conjugated dienes (1)
- Endothelial cells (1)
- Fenton reaction (1)
- Free fatty acid-iron complexes (1)
- Histochemistry (1)
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- Hydrogen peroxide (1)
- Hyperplastic smooth muscle (1)
- Initiation (1)
- Iron (1)
- Lipid hydroperoxide (1)
- Lipid peroxidation (1)
- Liposomes (1)
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- Neutrophils (1)
- Oxidative Stress (1)
- Oxoiron complexes (1)
- Perferryl Fe-oxo complexes (1)
- Perferryl iron (1)
- Smooth muscle (1)
- Superoxide (1)
- Trauma (1)
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Full-Text Articles in Engineering
Oxidative Mechanisms In Arterial Distension Injury: Observations Relevant To Restenosis After Angioplasty, Charles F. Babbs, Yuan Zhong
Oxidative Mechanisms In Arterial Distension Injury: Observations Relevant To Restenosis After Angioplasty, Charles F. Babbs, Yuan Zhong
Weldon School of Biomedical Engineering Faculty Working Papers
Background: To explore the hypothesis that tissue iron and reactive oxygen species, including superoxide (O2), mediate acute inflammatory and late hyperplastic responses to vascular injury, we studied experimental overdistension of normal carotid and femoral arteries in dogs. Experimental design: Arterial segments isolated in situ were distended with Ringer solution at 2 atmospheres pressure. In initial experiments the arteries were excised immediately after distension and immersed in diaminobenzidine solutions containing Mn++ ions to initiate histochemical reactions for O2. In other experiments distended arterial segments were reperfused with arterial blood in the presence or absence of the iron chelator, deferoxamine, or the …
Evidence That Free Fatty Acid-Iron Complexes Directly Initiate Lipid Peroxidation In Vitro And In Vivo: A New Mechanism Of Oxidative Stress, Steven C. Salaris, Charles F. Babbs, Joann Pham, John J. Turek
Evidence That Free Fatty Acid-Iron Complexes Directly Initiate Lipid Peroxidation In Vitro And In Vivo: A New Mechanism Of Oxidative Stress, Steven C. Salaris, Charles F. Babbs, Joann Pham, John J. Turek
Weldon School of Biomedical Engineering Faculty Working Papers
Through a series of biochemical and histochemical experiments we explored the novel hypothesis that iron and free fatty acids, liberated after tissue injury, combine to form liposoluble complexes that directly initiate lipid peroxidation. The addition of 100 M ferric iron to 30 mM linoleate suspensions at pH 7.4 produced time dependent lipid peroxidation, measured as conjugated diene formation. Complexes of 100 M ferric iron and 600 M pentanoate also initiated formation of conjugated dienes in linoleate suspensions and formation of malondialdehyde-like materials in rat liver slices. A histochemical stain for free fatty acids revealed positive reactions within cell membranes in …