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Network Analysis Of Intermediary Metabolism Using Linear Optimization. Ii. Interpretation Of Hybridoma Cell Metabolism, Joanne M. Savinell (Belovich), Bernhard O. Palsson

Chemical & Biomedical Engineering Faculty Publications

Analysis of metabolic networks using linear optimization theory allows one to quantify and understand the limitations imposed on the cell by its metabolic stoichiometry, and to understand how the flux through each pathway influences the overall behavior of metabolism. A stoichiometric matrix accounting for the major pathways involved in energy and mass transformations in the cell was used in our analysis. The auxiliary parameters of linear optimization, the so-called shadow prices, identify the intermediates and cofactors that cause the growth to be limited on each nutrient. This formalism was used to examine how well the cell balances its needs for …