Engineering Commons^™

High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Christopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittius, William N. Drohan

William H. Velander Publications

Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 pg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from …

Viscosities Of Vegetable Oils And Fatty Acids, Hossein Noureddini, B C. Teoh, L Davis Clements

Papers in Biomaterials

Data for viscosity as a function of temperature from 24 to llO°C (75 to 230°F) have been measured for a number of vegetable oils (crambe, rapeseed, corn, soybean, milk- coconut, lesquerella) and eight fatty acids in the from Cg to CZZ The viscosity measurements were performed according to ASTM test methods D 445 and D 446. Several correlations were fitted to the experimental data. Correlation constants for the best fit are presented. The range of temperature in which the correlations are valid is from 24'C (75'F), or the melting point of the substance, to llO°C (230°F). The correlation constants are …

Densities Of Vegetable Oils And Fatty Acids, Hossein Noureddini, B C. Teoh, L Davis Clements

Papers in Biomaterials

Complete data for density as a function of temperature have been measured for a number of vegetable oils (cramhe, rapeseed, corn, soybean, milkweed, coconut, lesquerella), as well as eight fatty acids in the range CS to C,, at temperatures from above their melting points to llQ°C (23Q°F). The specific gravity and density measure mente were performed according to American Society for Testing and Materials (ASTM) standard test methods D 368, D 891 and D 1298 for hydrometers and a modified ASTM D 369 and D 891 for pycnometers. Correlation constants, based on the experimental data, are presented for calculating the …

Sediment Delivery On Rill And Interrill Areas, John E. Gilley, D. C. Kincaid, W. J. Elliot, J. M. Laflen

Biological Systems Engineering: Papers and Publications

Equations which relate sediment delivery to a power function of flow rate and slope gradient were calculated in this study. The data used to parameterize the calculations were obtained from sites where crop residues had been removed. and moldboard plowing and disking had occurred. Measurements of sediment delivery resulting from simulated rainfall were obtained from preformed rills and interrill areas. The equations provided reliable sediment delivery estimates for selected soils located throughout the United States. To use the sediment delivery equations, soil-related parameter values must be identified. Multiple regression analyses were performed to relate parameter values used in the equations …

Simulated Crystalline Structures Of Aromatic Polyimides, Tze Wing Poon, B. David Silverman, Ravi F. Saraf, Angelo R. Rossi, Paul S. Ho

Ravi Saraf Publications

Semicrystalline structures involving molecular packing and optimized chain conformations of three polyimides have been obtained with the CHARMM moleculardynamics program, and their densities calculated. An eclipsed conformation of face-toface stacking of polymer chains with a relative shift is observed, and the shift is found to depend upon the molecular characteristics of the planar unit and chain linearity. Threedimensional structures obtained by calculating the optimized edge-on confirmation of the stacked units yield the smallest calculated density for PMDA-ODA, namely 1.515 g/cm³, followed by BPDA-PDA with 1.644 g/cm³ and PMDA-PDA with 1.712 g/cm³. Both PMDA-ODA and BPDA-PDA …

Beginning Of Motion For Selected Unanchored Residue Materials, John E. Gilley, Eugene R. Kottwitz

Biological Systems Engineering: Papers and Publications

Conservation tillage systems help to maintain residue materials from the previous crop on the soil surface. The potential for serious erosion may exist if crop residues are removed by overland flow. This study is conducted to identify the hydraulic conditions required to initiate residue movement by overland flow. Corn, cotton, peanut, pine needles, sorghum, sunflower, and wheat residue are placed in a flume on smooth and sand surfaces, and flow is then introduced in progressive increments. The discharge rate and flow velocity required to initiate residue movement are identified. Hydraulic measurements are used to calculate the ratio of critical flow …

Darcy-Weisbach Roughness Coefficients For Gravel And Cobble Surfaces, John E. Gilley, Eugene R. Kottwitz, Gary A. Wieman

Biological Systems Engineering: Papers and Publications

A laboratory study is conducted to measure Darcy-Weisbach roughness coefficients for selected gravel and cobble materials. Varying rates of flow are introduced into a flume in which a given size class of gravel or cobble material is securely attached. Roughness coefficients are calculated from measurements of discharge rate and flow velocity. The laboratory data are used to develop regression equations for relating roughness coefficients to surface cover and Reynolds number. The regression relations, which are developed for values of the Reynolds number from approximately 500 to 16,000, are tested using hydraulic data collected on surfaces containing a distribution of size …

Performance Of Chemigation Backflow Prevention Assemblies, H. M. Munir, Dean E. Eisenhauer, John E. Gilley

Biological Systems Engineering: Papers and Publications

When chemicals are applied using irrigation systems, there is potential for contamination of the water source by backflow if the irrigation system shuts off while unattended. This study was conducted to determine the operating and performance characteristics of new, i.e., unused, chemigation backflow prevention assemblies (CBPAs). Four manufacturer's models were tested in the laboratory. However, two of CBPAs have been modified since the testing was completed. In general, the CBPAs do not meet the standards established for municipal water suppliers and many industries. The backpressures required for the two current models to seal were 0.0 and 6.7 kPa (1.0 psi). …

Sediment Delivery On Rill And Interrill Areas, John E. Gilley, D. C. Kincaid, W. J. Elliot, J. M. Laflen

Biological Systems Engineering: Papers and Publications

Equations which relate sediment delivery to a power function of flow rate and slope gradient were evaluated in this study. The data used to parameterize the calculations were obtained from sites where crop residues had been removed, and moldboard plowing and disking had occurred. Measurements of sediment delivery resulting from simulated rainfall were obtained from preformed rills and interrill areas. The equations provided reliable sediment delivery estimates for selected soils located throughout the United States. To use the sediment delivery equations, soil-related parameter values must he identified. Multiple regression analyses were performed to relate parameter values used in the equations …

Darcy-Weisbach Roughness Coefficients For Gravel And Cobble Surfaces, John E. Gilley, Eugene R. Kottwitz, Gary A. Wieman

Department of Biological Systems Engineering: Conference Presentations and White Papers

A laboratory study is conducted to measure Darcy-Weisbach roughness coefficients for selected gravel and cobble materials. Varying rates of flow are introduced into a flume in which a given size class of gravel or cobble material is securely attached. Roughness coefficients are calculated from measurements of discharge rate and flow velocity. The laboratory data are used to develop regression equations for relating roughness coefficients to surface cover and Reynolds number. Accurate prediction of roughness coefficients for gravel and cobble surfaces will improve our ability to understand and properly model upland flow hydraulics.

Cultivators For Conservation Tillage, Robert Grisso, Paul J. Jasa, Elbert C. Dickey

Biological Systems Engineering: Papers and Publications

Cultivation, a proven weed control methods, should be used primarily to control weeds, rebuild ridges, or aerate poorly drained or crusted soils. Cultivating just to stir the soil usually dries the soil and can result in lower yields during drier cropping conditions. For example, cultivating during warm and windy conditions can result in ¼ to ¾ inches of soil moisture loss.

The main difference between a conservation tillage cultivator and a conventional cultivator is the ability to handle residue and penetrate the soil. By definition, conservation tillage should leave at least 30 percent of the soil surface covered with crop …

Management Of Feedlot Runoff Control Systems, Elbert C. Dickey, Gerald R. Bodman

Biological Systems Engineering: Papers and Publications

During recent years, most open feedlot operators have found it desirable and, in some cases, necessary to install runoff control facilities. Even though the primary purpose of feedlot runoff control systems is the prevention of water pollution, many producers have found that good management of these systems also offers limited economic benefits in the form of fertilizer nutrients and supplemental irrigation water. Well managed runoff control systems also reduce weeds, odors, insects and muddy areas at or near feedlot edges. The runoff control system that has proved most satisfactory for all areas of the country is one consisting of clean …

Definitions Of Tillage Systems For Corn, J. C. Siemens, Elbert C. Dickey, E. D. Threadgill

Biological Systems Engineering: Papers and Publications

If "tillage" is defined as the mechanical manipulation of soil, it follows, then, that a "tillage system" would be the sequence of soil-manipulation operations performed in producing a crop. Today, however, such a definition is recognized as inadequate. We know, for instance, that the management of non-harvested plant tissue (i.e., residue) affects both crop production and soil erosion, and that field operations in which the soil is not tilled have a marked influence on soil condition.

Therefore, in this publication, a tillage system is the sequence of all operations involved in producing the crop, including soil manipulation, harvesting, chopping or …