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Biomedical Engineering and Bioengineering
Endothelium; histochemistry; hydrogen peroxide; iron; ischemia; LDL; manganese; oxidants; reperfusion injury; superoxide
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Histochemical Demonstration Of Endothelial Superoxide And Hydrogen Peroxide Generation In Ischemic And Reoxygenated Rat Tissues, Charles F. Babbs, Meloney D. Cregor, Stephen F. Badylak
Histochemical Demonstration Of Endothelial Superoxide And Hydrogen Peroxide Generation In Ischemic And Reoxygenated Rat Tissues, Charles F. Babbs, Meloney D. Cregor, Stephen F. Badylak
Weldon School of Biomedical Engineering Faculty Publications
Objective: The aims were to test and evaluate two novel and independent histochemical methods for detecting the initial postischemic burst of superoxide and hydrogen peroxide in buffer perfused rat tissues during reflow after 60 min warm ischemia. Methods: The first is a high manganese/diaminobenzidine technique, in which superoxide oxidises Mn2+ to Mn3+, which in turn oxidizes diaminobenzidine to form amber colored polymers, observable by light microscopy. The second is a high iron/diaminobenzidine technique, in which hydrogen peroxide oxidizes diethylenetriaminepenta-acetate chelated Fe2+ to form intermediate species, which in turn oxidize diaminobenzidine similarly to Mn3+. Various isolated organs of the rat were …