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Theses/Dissertations

Chemical engineering

Biomedical Engineering and Bioengineering

Retrospective Theses and Dissertations

2006

Articles 1 - 3 of 3

Full-Text Articles in Engineering

Directed Growth And Selective Differentiation Of Neural Progenitor Cells Using A Synergistic Combination Of Topographical And Soluble Cues , Jennifer Behr Recknor Jan 2006

Directed Growth And Selective Differentiation Of Neural Progenitor Cells Using A Synergistic Combination Of Topographical And Soluble Cues , Jennifer Behr Recknor

Retrospective Theses and Dissertations

The purpose of this study is to develop strategies to facilitate nerve regeneration using a synergistic combination of guidance cues. We are investigating the cellular mechanisms of development using adult rat hippocampal progenitor cells (AHPCs) and have demonstrated that manipulating a combination of physical, chemical and biological cues can lead to oriented astrocyte and neural progenitor cell outgrowth and can influence progenitor cell differentiation. To provide physical guidance, micropatterned polystyrene (PS) substrates were fabricated and chemically modified with laminin. Astrocytes or AHPCs cultured on these substrates align along the grooves of the patterned surface extending highly elongated processes. To further ...


Recovery And Purification Of Recombinant Protein From Transgenic Corn By Aqueous Two-Phase Partitioning , Zhengrong Gu Jan 2006

Recovery And Purification Of Recombinant Protein From Transgenic Corn By Aqueous Two-Phase Partitioning , Zhengrong Gu

Retrospective Theses and Dissertations

Aqueous two-phase (ATP) partitioning was paired in a variety of ways to use of transgenic corn for protein expression. Systems were developed to selectively partition corn proteins from classes of potential recombinant proteins as well as the specific case of dog gastric lipase. More fundamentally, ATP partitioning was used to expand 2D gel electrophoretic characterization of protein size and pI to the third dimension of hydrophobicity with the usefulness of the method illustrated for corn extracts;ATP partitioning in polyethylene glycol (PEG)-salt systems separates proteins primarily according to their surface hydrophobicity differences. The average hydrophobicity of pH 7 extracts ...


Engineering Polyanhydride Microspheres For The Stabilization And Controlled Release Of Proteins , Amy Sywassink Determan Jan 2006

Engineering Polyanhydride Microspheres For The Stabilization And Controlled Release Of Proteins , Amy Sywassink Determan

Retrospective Theses and Dissertations

This work investigates the use of polyanhydride microspheres as drug delivery carriers for therapeutic proteins. The polymers investigated are poly(sebacic anhydride) and opolymers of poly[1,6-bis(p-carboxyphenoxy)hexane] (poly(CPH)) and poly(SA), 20:80 (CPH:SA), 50:50 (CPH:SA), and 80:20 (CPH:SA). The model protein bovine serum albumin (BSA) was encapsulated in poly(SA), 20:80 (CPH:SA), 50:50 (CPH:SA), and 80:20 (CPH:SA) microspheres to determine the feasibility of using polyanhydrides as protein carriers. Poly(SA) and 20:80 (CPH:SA) were found to stabilize the encapsulated BSA and were ...