Engineering Commons^™

Aligning Anatomical Knee Model With Kinematic Experimental Data To Investigate Squat Exercise, Jose Mario Salinas

Theses and Dissertations

A two-dimensional inverse dynamics anatomical model of the human knee was developed by aligning anatomical structures of the knee (taken from x-rays) with kinematic experimental data (collected from motion capture cameras). The mathematical model was used to study the forces related to the tibiofemoral and patellofemoral joints during the squat and knee extension exercises. Forces calculated by the model included: tibiofemoral contact force, patellofemoral contact force, forces generated by cruciate ligaments, quadriceps force, and patellar tendon force. Bone alignment consisted of superimposing the geometric center of the lateral femoral condyle (from x-rays) with the relative instantaneous center of rotation of …

Multi-Objective Design Optimization Of Reverse Total Shoulder Arthroplasty To Maximize Range Of Motion And Joint Stability, Josie A. Elwell

Graduate Dissertations and Theses

Reverse total shoulder arthroplasty was developed to restore range of motion (ROM) and joint stability to patients with pre-operative conditions that are not addressed by conventional replacements. Although reverse total shoulder arthroplasty is the current gold standard for treating a range of indications, the effects of varying its design on functional outcomes of the procedure are still not well understood.

To that end, it is not yet clear which configurations, in terms of both design and surgical placement parameters, maximize range of motion and stability of the joint. It was hypothesized that there is trade-off between the two. These types …

Design Of A Multidirectional Wear Testing Device For Simulating Wear Of Biocompatible Materials Used In Joint Implants, Carlos A. Rodriguez Betancourth

Theses and Dissertations

In the present work, a novel multidirectional pin-on-disc wear testing device designated CNC-POD (computer-numerical-control pin-on-disc) capable of replicating the cross-shear motions experienced by a prosthesis in vivo was designed and developed in the UTRGV in order to evaluate the wear resistance of new biomaterials. The CNC-POD consists of six temperature-controlled pin-on-disc stations mounted on a two-axis CNC table (X-Y). Each pin-on-disc station is load-programmable up to 510 Newtons (115 pounds). The CNC table allows the machine to reproduce a wide range of 2D patterns in millimeter scale on the six stations under a specific load. Friction force is measured during …

Development Of A Multiband Remote Sensing System For Determination Of Unsaturated Soil Properties, Cyrus D. Garner

Graduate Theses and Dissertations

A multiband system including active microwave sensing and visible-near infrared reflectance spectroscopy was developed to measure unsaturated soil properties in both field and laboratory environments. Remote measurements of soil volumetric water content (θv), soil water matric potential (ψ), and soil index properties (liquid limit [LL], plastic limit [PL], and clay fraction [CF]) were conducted. Field-based measurement of θv was conducted using a ground-based radar system and field measurements within 10 percentage points of measurements acquired with traditional sampling techniques were obtained. Laboratory-based, visible and near infrared spectroscopy was found to be capable of obtaining empirical, soil specific regression functions (partial …

Temporal Evaluation Of Corn Respiration Rates Using Pressure Sensors, Gagandeep Singh Ubhi

Graduate Theses and Dissertations

High respiration rate of a grain indicates faster degradation of its dry matter. Proper grain management requires chronological and precise measurements of carbon dioxide evolved from grain respiration during the postharvest storage duration. Therefore the main goal of this research was to develop a new technique that evaluates temporal corn respiration rate using pressure sensors. It was based on measuring pressure drop associated with the grain respiration in a closed container and using it to calculate the grain respiration rates.

Dry corn (Zea Mays L.) was procured from a local farmer and stored at 4ºC. Corn rewetting technique was applied …

Development Of A One Pass Microwave Heating Technology For Rice Drying And Decontamination, Deandrae Lynette Smith

Graduate Theses and Dissertations

An industrial microwave (MW) system operating at 915 MHz frequency was used to dry high moisture content (MC) (23% to 24% wet basis) medium-grain rough rice samples (cv. Jupiter). The rice beds were contained in a modified tray that accommodated up to 9 kg of rice separated by thin fiberglass mesh in 3 kg increments. Each layer of rice was fitted with fiber optic sensors connected to a real time data logger during MW treatments. It was determined that drying rice to a MC of 14% to 16% was feasible with the application of MW specific energy at 600 kJ/kg-grain …

Improvement Of Fluorescence-Based Microfluidic Dna Analyzers, Collin Tranter

Doctoral Dissertations

A tremendous effort continues in the development of micro-total-analysis-systems; in support of this, many chemical passivation methods have been developed to enhance the biocompatibility of such microfluidic systems. However, the suitability of these passivation techniques to many fluorescence-based assays still remains inconsistent. This part of this work is focused on the performance of a third generation intercalating DNA dye when used within microfluidic devices treated with a select variety of passivating coatings. The results of these tests indicate that passivation coatings which are intended to shed DNA based on electrostatic repulsion will in fact imbibe the fluorescent DNA intercalating dye …

Characterizing The Effects Of Repetitive Head Trauma In Female Soccer Athletes For Prevention Of Mild Traumatic Brain Injury, Diana Otero Svaldi

Open Access Dissertations

As participation in women’s soccer continues to grow and the longevity of female athletes’ careers continues to increase, prevention of mTBI in women’s soccer has become a major concern for female athletes as the long-term risks associated with a history of mTBI are well documented. Among women’s sports, soccer exhibits the highest concussion rates, on par with those of men’s football at the collegiate level. Head impact monitoring technology has revealed that “concussive hits” occurring directly before symptomatic injury are not predictive of mTBI, suggesting that the cumulative effect of repetitive head impacts experienced by collision sport athletes should be …

Design And Plan Of A Modified Hydroponic Shipping Container For Research, John A. Houtman

Open Access Theses

As the world’s population continues to increase, food production will need to increase in order to meet the predicted rise in food demand. However, with increased pressure on cropland available from environmental effects and urbanization, new innovative methods of crop production need to be researched in order to increase agricultural production with limited land. This research focuses on the design of a single form of urban agriculture that is considered Zfarming and has the potential to produce quality urban agricultural produce through ground-based measures. This project produced detailed step-by-step analysis of the design process, develop variability within the modified hydroponic …

Mathematical Analysis Of Feedback Targets Of Bmp Signaling In Drosophila Embryonic Development, Yan Luo

Open Access Theses

Bone morphogenetic proteins (BMPs) drive a range of cellular processes especially in the early stages of embryonic development. This family of proteins acts as one of the most important extracellular signals in development pattern formation across the animal kingdom. Cells in embryos differentiate into different cell types in response to the concentration level of BMP. This complex process is regulated by multiple regulators that serve to tune the signal response.

Extensive experimental and computational research has been performed to analyze BMP regulation in Drosophila, a widely studied model organism, and has advanced our understanding of animal development. Because of …

Knee Joint Internal Forces During Squat Jump Exercise, Ricardo Moreno

Theses and Dissertations

The purpose of this research is to investigate muscular ligament and joint contact forces produced during squat jump exercise. An inverse dynamics, two dimensional, leg model is used to describe the motion in the sagittal plane. The lower extremity model includes two bones, tibia and femur, tibio-femoral ligaments, and muscles such as quadriceps, hamstrings, and gastrocnemius. The ligaments are anatomically modeled as nonlinear strings, but the femoral condyle is modeled as a circle and the tibial plateau as a straight line. Experimental squat jump exercises are conducted to obtain the ground reaction forces, the angular accelerations, and centroid linear acceleration …

Exploring The Production Of Extracellular Matrix By Astrocytes In Response To Mimetic Traumatic Brain Injury, Addison Walker

Graduate Theses and Dissertations

Following injury to the central nervous system, extracellular modulations are apparent at

the site of injury, often resulting in a glial scar. Astrocytes are mechanosensitive cells, which can create a neuroinhibitory extracellular environment in response to injury. The aim for this research was to gain a fundamental understanding of the affects a diffuse traumatic brain injury has on the astrocyte extracellular environment after injury. To accomplish this, a bioreactor culturing astrocytes in 3D constructs delivered 150G decelerations with 20% biaxial strain to mimic a traumatic brain injury. Experiments were designed to compare the potential effects of media type, number of …

Examination Of Pseudomonas Fluorescence As A Recombinant Expression Host: Cloning, Expression, And Chromatography, Ahmed K.Ali Elmasheiti

Graduate Theses and Dissertations

In an effort to expand the pool of bacterium useful for biotechnology applications, Pseudomonas fluorescens, a common gram negative microbe, was examined for its ability to function in a recombinant setting. P. fluorescens is ubiquitous in nature and was initially identified as a soil bacterium found in dirt and is typically associated with plant material. Past literature indicates that it shared characteristics common to Escherichia coli and Bacillus subtilis, including simple growth conditions and potential cloning vectors, providing motivation to look into both the upstream and downstream characteristics of this bacterium. First, it was demonstrated that P. fluorescens could be …

A Portable And Automatic Biosensing Instrument For Detection Of Foodborne Pathogenic Bacteria In Food Samples, Zhuo Zhao

Graduate Theses and Dissertations

Foodborne diseases are a growing public health problem. In recent years, many rapid detection methods have been reported, but most of them are still in lab research and not practical for use in the field. In this study, a portable and automatic biosensing instrument was designed and constructed for separation and detection of target pathogens in food samples using nanobead-based magnetic separation and quantum dots (QDs)-labeled fluorescence measurement. The instrument consisted of a laptop with LabVIEW software, a data acquisition card (DAQ), a fluorescent detector, micro-pumps, stepper motors, and 3D printed tube holders. First, a sample in a syringe was …

A Bifunctional Nanocomposites Based Electrochemical Biosensor For In-Field Detection Of Pathogenic Bacteria In Food, Meng Xu

Graduate Theses and Dissertations

This research focused on the application of electrochemical biosensors for the rapid detection of pathogenic bacteria, Escherichia coli O157:H7 and Salmonella Typhimurium, in foods. The possible presence of pathogenic bacteria in foods has always been a great threat to the wellbeing of people and the revenue of food companies. Therefore, the demand for rapid and sensitive methods to detect foodborne pathogens is growing. In this research, an impedimetric immunosensor was first developed for the rapid detection of E. coli O157:H7 and S. Typhimurium in foods. It was based on the techniques of immunomagnetic separation, enzyme labelling, and electrochemical impedance spectroscopy …

Generalized Partial Directed Coherence And Centrality Measures In Brain Networks For Epileptogenic Focus Localization, Joshua Aaron Adkinson

Doctoral Dissertations

Accurate epileptogenic focus localization is required prior to surgical resection of brain tissue for treatment of patients with intractable temporal lobe epilepsy, a clinical need that is partially fulfilled to date through a subjective, and at times inconclusive, evaluation of the recorded electroencephalogram (EEG). Using brain connectivity analysis, patterns of causal interactions between brain regions were derived from multichannel EEG of 127 seizures in nine patients with focal, temporal lobe epilepsy (TLE). The statistically significant directed interactions in the reconstructed brain networks were estimated from three second intracranial multi-electrode EEG segments using the Generalized Partial Directed Coherence (GPDC) and validated …

Evaluating The Two-Stage Ditch As A New Best Management Practice, Andi Hodaj

Open Access Dissertations

A two-stage ditch involves modifications of a traditional drainage ditch to resemble more the features of a natural stream. The idea is to create or simulate extended benches on both sides of the ditch that would develop naturally over a period of time in a stream because of geomorphological processes. Previous research in Indiana and Ohio has shown that two-stage ditches offer the potential to reduce sediment load and extend the interaction time between water and vegetation on the benches allowing larger uptake of nutrients from the vegetation on the extended benches, and increasing the denitrification rates in the bench …

The Role Of Mechanical Loading In Chondrocyte Signaling Pathways, Qiaoqiao Wan

Open Access Dissertations

Chondrocytes are a predominant cell type present in articular cartilage, whose integrity is jeopardized in joint degenerative diseases such as osteoarthritis (OA). In the chondrocytes of patients with OA, the elevated levels of inflammatory cytokines such as interleukin 1β (IL1β) and tumor necrosis factor α (TNFα) have been reported. These cytokines contribute to degradation of cartilage matrix by increasing activities of proteolytic enzymes. In addition to their contribution to proteolytic enzymes, these cytokines adversely affect anabolic activity of chondrocytes by inhibiting the production of proteoglycans and type II collagen. Therefore, blocking the action of these cytokines is a potential strategy …

Mapping And Analyzing Energy Use And Efficiency In A Modified Hydroponic Shipping Container, Rachel E. Sparks

Open Access Theses

In urban centers today, vertical farming is becoming a popular alternative to conventional agriculture in an effort to increase local food production and improve urban food security by growing crops using hydroponic methods in controlled environment spaces. More specifically, one vertical method involves growing crops inside refurbished shipping containers, or a “farm-in-a-box” concept, which offers a flexible, mobile, and scalable means of year-round food production in a variety of climates. Despite benefits of producing food locally, some of the concerns associated with these vertical farming systems include high energy consumption from climate control and electric lighting systems as well as …

Biocompatible Microchannel Scaffold With Microwires For Recording Regenerative Peripheral Nerve Neural Spikes, Manisankar Chennamsetti

Theses and Dissertations

A new process for the fabrication of a microchannel scaffold with microwires for peripheral nerve applications is presented. This microchannel scaffold implemented between the ends of nerves, the axons of which regenerate through microchannel in scaffold and fixed microelectrodes. This device is entirely handcrafted using commercially available materials such as microwires, PDMS film, liquid PDMS, dental cement, and epoxy glue. This device was implemented in the a Lewis rat sciatic nerve to better analyze the electrical signals of regenerated axons. 64-electrode microchannel scaffolds were developed for both peripheral nerve interfacing and peripheral nerve regeneration. The microwires were used for recording …

Nano Clay-Enhanced Calcium Phosphate Cements And Hydrogels For Biomedical Applications, Udayabhanu Jammalamadaka

Doctoral Dissertations

Biomaterials are used as templates for drug delivery, scaffolds in tissue engineering, grafts in surgeries, and support for tissue regeneration. Novel biomaterial composites are needed to meet multifaceted requirements of compatibility, ease of fabrication and controlled drug delivery. Currently used biomaterials in orthopedics surgeries suffer limitations in toxicity and preventing infections. Polymethyl methacrylate (PMMA) used as bone cement suffers from limitations of thermal necrosis and monomer toxicity calls for development of better cementing biomaterials. A biodegradable/bioresorbable cement with good mechanical properties is needed to address this short coming. Metal implants used in fixing fractures or total joint replacement needs improvements …

Microwire Regenerative Peripheral Nerve Interfaces With Wireless Recording And Stimulation Capabilities, Ali Ajam

Theses and Dissertations

A scalable microwire peripheral nerve interface was developed, which interacted with regenerated peripheral nerves in microchannel scaffolds. Neural interface technologies are envisioned to facilitate direct connections between the nervous system and external technologies such as limb prosthetics or data acquisition systems for further processing. Presented here is an animal study using a handcrafted microwire regenerative peripheral nerve interface, a novel neural interface device for communicating with peripheral nerves. The neural interface studies using animal models are crucial in the evaluation of efficacy and safety of implantable medical devices before their use in clinical studies.16-electrode microwire microchannel scaffolds were developed for …

Experimental And Computational Studies Of Cortical Neural Network Properties Through Signal Processing, Wesley Patrick Clawson

Graduate Theses and Dissertations

Previous studies, both theoretical and experimental, of network level dynamics in the cerebral cortex show evidence for a statistical phenomenon called criticality; a phenomenon originally studied in the context of phase transitions in physical systems and that is associated with favorable information processing in the context of the brain. The focus of this thesis is to expand upon past results with new experimentation and modeling to show a relationship between criticality and the ability to detect and discriminate sensory input. A line of theoretical work predicts maximal sensory discrimination as a functional benefit of criticality, which can then be characterized …

Handcrafted Electrocorticography Electrodes For A Rodent Neural Activity, Nishat Tasnim

Theses and Dissertations

Electrocorticography (ECoG) is a minimally invasive neural recording method that has been extensively used for neuroscience applications. Although many neural recording methods exist, ECoG provides a combination of stability, high spatial and temporal resolution with chronic and mobile capabilities. This paper presents an animal study using a low cost and simple handcrafted ECoG electrode that is made of commercially accessible materials. The study is performed on a Lewis rat implanted with a handcrafted 32-channel non-penetrative ECoG electrode covering an area of 3 x 3 mm 2 on the cortical surface. The ECoG electrodes were placed on the motor and somatosensory …

Genotoxicity Of Graphene In Escherichia Coli, Ananya Sharma

Graduate Theses and Dissertations

Rapid advances in nanotechnology necessitate assessment of the safety of nanomaterials in the resulting products and applications. One key nanomaterial attracting much interest in many areas of science and technology is graphene. Graphene is a one atom thick carbon allotrope arranged in a two-dimensional honeycomb lattice. In addition to being extremely thin, graphene has several extraordinary physical properties such as its exceptional mechanical strength, thermal stability, and high electrical conductivity. Graphene itself is relatively chemically inert and therefore pristine graphene must undergo a process called functionalization, which is combination of chemical and physical treatments that change the properties of graphene, …

Assessment Of An Industrial Type Radiant Heating System For Drying Rough Rice, Anne Adhiambo Okeyo

Graduate Theses and Dissertations

Rough rice is normally harvested at moisture contents (MCs) higher than that for safe storage. The objective for this study was to investigate the effectiveness of scaled-up infrared (IR) heating followed by tempering steps to dry freshly-harvested rough rice. An industrial type, pilot scale, IR drying equipment, designed to convey rough rice on a vibrating conveyor belt was used in this study. The heating zone of the equipment had catalytic IR emitters powered by natural gas. Freshly-harvested rough rice of long-grain pureline (cv. Cheniere), long-grain hybrid (cv. 6XP 756) and medium-grain (cv. CL 271) cultivars at initial moisture contents (IMCs) …

Is Metabolism Goal-Directed? Investigating The Validity Of Modeling Biological Systems With Cybernetic Control Via Omic Data, Frank T. Devilbiss

Open Access Dissertations

Cybernetic models are uniquely juxtaposed to other metabolic modeling frameworks in that they describe the time-dependent regulation of cellular reactions in terms of dynamic "metabolic goals." This approach contrasts starkly with purely mechanistic descriptions of metabolic regulation which seek to explain metabolic processes in high resolution — a clearly daunting undertaking. Over a span of three decades, cybernetic models have been used to predict metabolic phenomena ranging from resource consumption in mixed-substrate environments to intracellular reaction fluxes of intricate metabolic networks. While the cybernetic approach has been validated in its utility for the prediction of metabolic phenomena, its central feature, …

Influence Of The 3d Microenvironment On Glioblastoma Migration And Drug Response, Ruth Marisol Herrera Perez

Open Access Dissertations

Glioblastoma (GBM) is a highly invasive brain cancer characterized by poor prognosis. Despite significant efforts by the basic and clinical research community our understanding of GBM progression and recurrence has been incremental. Improvements in therapeutic response have been dismal, and GBM continues to be the deadliest tumor of the central nervous system, with patient average survival rate of 12 months. Synergistic relationships that the tumor cells establish with the brain microenvironment have been proven fundamental for successful tumor progression and maintenance. Yet, many in vitro GBM studies are performed in formats that fail to recapitulate the most essential component of …

Consonant Discrimination In The Inferior Colliculus Of Young And Aged Rats, Christopher S. Soverns

Open Access Theses

Complex acoustic stimuli are thoroughly encoded and processed along the primary auditory pathway to give reliable and relevant information about the environment, and elucidating the neural coding mechanisms is essential to informing clinical attempts to combat auditory dysfuntion. Receiving a uniquely diverse set of ascending and descending inputs, the inferior colliculus (IC) is a site of intricate temporal processing. In this work, natural and modified human speech is used to investigate discrimination of voice onset time (VOT) in the spiking output of IC neurons. A template-matching classification model is proposed in which single stimulus presentation responses are correlated with aggregate …

Clay Nanotube Composites For Antibacterial Nanostructured Coatings, Christen J. Boyer

Doctoral Dissertations

A surging demand for the development of new antimicrobial nanomaterials exists due to the frequency of medical device-associated infections and the transfer of pathogens from highly touched objects. Naturally occurring halloysite clay nanotubes (HNTs) have shown to be ideal particles for polymer reinforcement, time-release drug delivery, nano-reactor synthesis, and as substrate material for nanostructured coatings.

This research demonstrates the feasibility of a novel method for coating HNTs with metals for antibacterial applications. The first ever ability to coat HNTs through electrolysis was developed for customizable and multi-functional antibacterial nanoparticle platforms. HNTs were investigated as substrate for the deposition of copper …