Engineering Commons^™

A Multi-Channel 3d-Printed Bioreactor For Evaluation Of Growth And Production In The Microalga Dunaliella Sp, Cristian A. Cox

Electronic Theses and Dissertations

We explored the capabilities of additive manufacturing using a photo-cured jetted material 3D printer to manufacture a milli-microfluidic device with direct application in microalgae Dunaliella sp growth and intracellular compounds biosynthesis tests. A continuous microbioreactor for microalgae culture was CAD designed and successfully built in 1 hour and 49 minutes using black photopolymer cured by UV and a support material. The microreactor was made up of 2 parts including the bioreactor itself and a microchannel network for culture media fluids and microalgae. Both parts were assembled to form a single unit. Additional optical and auxiliar components were added. An external …

Abe Fermentation From Low Cost Substrates, Kai Gao

Electronic Thesis and Dissertation Repository

The high cost of substrate and product inhibition in the fermentation broth remains two major problems associated with bio-butanol production. This thesis aims to solve these problems by examining abundant lignocellulosic biomass as potential feedstocks and exploring novel substrates such as carbohydrates derived from microalgae for ABE fermentation. The commonly observed toxic effect after pretreatment of lignocelluslosic biomass was removed by resin adsorption, where the resin could also serve as an in-situ butanol recvoery devices.

Corn cobs (an agricultural waste), switchgrass (an energy crop) and phragmites (an invasive plant in North America) were investigated as substrates for ABE fermentation by …

Direct Conversion Of Wet Microalgae And Oleaginous Yeast To Biodiesel Using Ionic Liquids, Valerie Orr

Electronic Thesis and Dissertation Repository

This thesis examined the development of an oleaginous yeast or microalgae based biorefinery process. First, improvements were made to the Nile Red assay, a high-throughput method for monitoring lipid accumulation in oleaginous organisms resulting in a significantly more reproducible and accurate assay. This assay was then used to optimize lipid production during heterotrophic cultivation of microalgae on glucose using response surface methodology resulting in microalgae with high lipid content (37.6% wt.). In order to improve the renewability of heterotrophic lipid production, both oleaginous yeast and microalgae were cultivated using pyrolytic sugars, produced via fast pyrolysis of pinewood waste. The effects …

Microalgae Sorption Of Ten Individual Heavy Metals And Their Effects On Growth And Lipid Accumulation, Eric M. Torres

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study is to determine the potential microalgae have for being a heavy metal remediation tool. Heavy metals are naturally occurring elements that can be found inside the earth or on its surface. Typically, heavy metals are found naturally at low concentrations. However, through human activities, heavy metals are continually making their way to the surface and being concentrated. This happens largely through mining and manufacturing operations. For example, NiCd batteries produce waste containing nickel and cadmium, and paper manufacturing produces waste containing high levels of lead. As underdeveloped nations continue to industrialize and the world population …

Impact Of Heavy Metal Contamination From Coal Flue Gas On Microalgae Biofuel And Biogas Production Through Multiple Conversion Pathways, Derek E. Hess

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Microalgae has a great potential to help alleviate the world's current dependence on fossil fuels. Microalgae is a single celled organism that produces lipids as a food storage device much like humans and animals store fat. These lipids are a kind of oil that when harvested can be utilized as biofuel.

As the technology surrounding using microalgae as a source of biofuel has advanced key bottlenecks in the eventual large-scale production of microalgae based biofuel have been discovered. One of these bottlenecks is the need to cheaply supply carbon for microalgae growth. One idea that has emerged as a plausible …

Behavior Of Light In A Photobioreactor And Design Of Light Guides, Anand Rajendran

Electronic Theses and Dissertations

The purposes of the thesis is to explain the microalgae cultivation in a photobioreactor system; demonstrate the light activity in a flat-plate photobioreactor; and design the light channeling system. Generally, microalgae are cultivated in open ponds and in closed photobioreactor systems. In open ponds, the productivity of microalgal biomass is very low, due to lack and improper use of carbon and energy sources. In photobioreactor systems, all required inputs can be supplied effectively to the microalgae which in turn increases the biomass productivity. However, these systems are expensive to build and to maintain in a largescale basis. In a photobioreactor, …