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Full-Text Articles in Engineering
Retention Of Cellular Viability And Growth Potential In Cryopreserved Bovine Arterial Tissue: Implications For Clinical Use Of Cryopreserved Vascular Allografts, Kip G. Gardner
Biological Sciences Theses & Dissertations
Segments of bovine calf aortas and pulmonary arteries were cryopreserved at -196° C for two weeks to 18 months and subsequently thawed. Cellular morphology, viability, and growth potential in these segments were then compared with the same attributes in segments from fresh (control) arteries. Scanning electron microscopy revealed no disruption of intimal surfaces attributable to cryopreservation. Endothelial cells were successfully cultured from all cryopreserved arterial segments. Endothelial monolayers grown from cryopreserved arteries had a cobblestone appearance and expressed Factor VIII antigen but not smooth muscle alpha-actin. Plating efficiency in primary cultures of endothelial cells was lower for cryopreserved arteries than …
The Effects Of Twist On Pressure Through A Teflon Vascular Prosthesis, Steven Alfred Olivieri
The Effects Of Twist On Pressure Through A Teflon Vascular Prosthesis, Steven Alfred Olivieri
Theses
A closed loop fluid system was developed which mimics the flow curve in the human circulatory system in order to test the effects of pressure drop and flow velocity on artificial arterial implants. This device consists of a piston pump connected to a microcomputer controlled servo-motor. Fluid (in this case distilled water) flows through transparent Tygon tubing to a test chamber. The prosthetic tube is placed in the fluid but is attached through a specially constructed chamber. The pressure across the implant can be measured for different conditions. System compliance was generated by using flexible tubing in the return loop …