Engineering Commons^™

Methods To Increase Algae Biomass Productivity In Raceway Pond Monocultures, Ryan S. Anderson

Master's Theses

The economics of algae biofuels and bioproducts would be improved by increased biomass productivity. Two studies on this potential are described in this thesis – one on a locally isolated filamentous yellow-green alga and the other on a planktonic strain genetically improved via selective enrichment.

Polycultures have been viewed as productive, stable, and, in some cases, harvestable by natural bioflocculation. Local native strains might have higher productivity than culture collection strains because they are already adapted to local outdoor conditions. In this study, the filamentous yellow-green alga Tribonema minus was isolated from a local volunteer polyculture. Its productivity as a …

A Study On The Simultaneous Nitrification And Denitrification Process Of A Membrane Aerated Bioreactor Augmented By Biowish Aqua, Gavrielle Orman

Master's Theses

Nitrogen pollution is a growing problem that is detrimental to the environment and the economy. Traditional treatment of nitrogen is a multi-stage process, expensive, operationally intensive, and requires large land areas. This research studied the effects of BiOWiSH® Aqua (Aqua), a biological enhancement product, on the simultaneous nitrification and denitrification process in a membrane aerated bioreactor (MABR) to determine if it is a feasible application for wastewater treatment. The MABR used during experimentation was a small-scale batch reactor with a continuous flow of air through a silicone membrane.

The effect of carbon source and concentration on nitrogen removal rates and …

Wastewater Treatment And Nitrogen Removal In Bench-Scale Photobioreactors Operated With Solids Recycling, Erik Hoffnagle

Master's Theses

This thesis examines the effect of solids recycling on nitrification, organic carbon removal, and algal-bacterial productivity in bench-scale photobioreactors (PBRs) simulating winter pond conditions in San Luis Obispo, California and a 6-acre raceway system in Delhi, California. Two sets of duplicate photobioreactors operated with or without solids recycling were fed primary clarifier effluent (1^oEff) for the first experiment, and facultative pond effluent (FAC) for the second experiment.

In both experiments mean productivity was lower in solids recycled PBRs (3.67 and 2.3 g/m²-day), than in controls without solids recycling (4.15 and 3.9 g/m²-day). When fed …

Nutrient Removal In Microalgae Raceway Ponds And Nitrification Modeling, Esmeralda Diego

Master's Theses

This thesis explores the treatment of municipal wastewater using pilot-scale raceway ponds and looks specifically at the capability of the raceways in removing BOD and nitrogen. Nine 33 square-meter algal raceway ponds were used to conduct research at the San Luis Obispo Water Resources Recovery Facility. Main objectives of this study were to increase the removal of total ammonia nitrogen (NH₃-N plus NH₄⁺-N) from municipal wastewater through increased assimilation and nitrification. Raceway ponds with CO₂ addition were operated in series with an intermediate settling step and a total hydraulic retention time (HRT) of 4 …

Effect Of Bioaugmentation Product Biowish® Aqua™ On Nitrogen Removal In Wastewater, Patrick Cassidy Kalvass

Master's Theses

Biological nutrient removal (BNR) from wastewater, and specifically nitrogen removal, is a growing concern to wastewater dischargers such as municipalities. Excess nutrients in effluent can create problems such as eutrophication, toxicity to aquatic life, and dissolved oxygen depletion in receiving waters. BNR systems have been installed in many locations with success, but their operation presents operational and financial demands greater than conventional biological treatment. Nitrogen removal is typically performed in sequential autotrophic nitrification and denitrification, which increases needed energy input, operational complexity, and therefore cost. Simultaneous nitrification-denitrification (SNdN) achieved in a single system has also been successfully implemented, however operational …

Production And Harvest Of Microalgae In Wastewater Raceways With Resource Recycling, Alexander Colin Roberts

Master's Theses

Microalgae can be grown on municipal wastewater media to both treat the wastewater and produce feedstock for algae biofuel production. However the reliability of treatment must be demonstrated, as well as high areal algae productivity on recycled wastewater media and efficient sedimentation harvesting. This processes was studied at pilot scale in the present research.

A pilot facility was operated with nine CO₂-supplemented raceway ponds, each with a 33-m² surface area and a 0.3-m depth, continuously from March 6, 2013 through September 24, 2014. The ponds were operated as three sets of triplicates with two sets continuously fed …

Investigation Of A Commercial Product (Biowish™) For Nitrogen Management, Eva Lee

Master's Theses

Abstract

Investigation of a Commercial Product (BiOWiSH­­^TM) for Nitrogen Management

Eva Lee

BiOWiSH–Aqua, which has the capability of treating nitrogen from wastewater through bioaugmentation, is a commercial product consisting of a blend of microorganisms developed by BiOWiSH Technologies. A study of the treatment of nitrogen compounds (i.e. , , and ) using Biowish–Aqua was conducted using small scale experiments (flask experiments) and large scale experiments (column reactor experiments). In this work, column reactors were created to test Biowish–Aqua’s nitrogen treating capabilities by providing enough depth to simulate the dissolved oxygen gradient that can be observed in a pond. …

Dissolved Organic Matter Characterization And Impact On Mercury Binding In An Urbanizing Watershed, Mykel Mendes

Master's Theses

Dissolved organic matter (DOM) is ubiquitous in aquatic ecosystems, interacting with and controlling the bioavailability and transport of mercury (Hg) and other trace metals through complexation. The complexation of ionic Hg (Hg²⁺) with DOM can either limit methyl mercury (MeHg) bioavailability or increase MeHg production by stimulating microbial growth. While substantial investigations have examined Hg-DOM interactions in forested ecosystems, little research has been conducted on the Hg-DOM relationship within an urbanized river setting subjected to various anthropogenic sources. The various runoff and pollutant sources vary in DOM quality and character. Three sources of organic matter from the North …

Comprehensive Analysis Of Effluent Organic Matter From Five Wastewater Treatment Plants In Connecticut And Comparison To Natural Organic Matter, Matthew L. Quaranta

Master's Theses

Natural organic matter is an important driver of biotic and abiotic processes in aquatic environments. Wastewater treatment plants discharge a substantial amount of organic matter into the environment; however effluent organic matter has not been well studied. In this study, traditional organic geochemical techniques were applied to characterize effluent organic matter. Effluent organic matter was isolated by DAX8 (hydrophobic fraction) and XAD4 (transphilic fraction) Amberlite resins. Extraction efficiencies of effluent organic matter by DAX8 resins ranged from 18 to 42 percent as a result of larger content of hydrophilic organic matter than natural organic matter. Average organic matter molecular weights …

Bioflocculation For Control Of Wastewater Pond Microalgae, Daniel Thomas Frost

Master's Theses

Investigates several hypotheses regarding the use of bioflocculation as a harvesting method for wastewater pond microalgae. Research performed on pilot-scale high rate ponds (HRPs) on the California Central Coast.

Lipid Productivity Of Algae Grown On Dairy Wastewater As A Possible Feedstock For Biodiesel, Ian C. Woertz

Master's Theses

The objective of this thesis is to develop a biological wastewater treatment system that utilizes algal growth to simultaneously create renewable energy in the form of biodiesel and digester biogas, remove polluting nutrients, and abate greenhouse gases. Research under the Department of Energy Aquatic Species Program during 1978-1996 concluded that cultivating algae for biofuels was cost prohibitive at that time and that an integrated approach should be studied that combined wastewater treatment with algal biofuel production. Nutrient removal, in particular nitrogen and phosphorus, from wastewater is a growing regulatory need and the use of algae cultivation could create a unique …