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Volumetric Imaging Using The Pupil-Matched Remote Focusing Technique In Light-Sheet Microscopy, Sayed Hassan Dibaji Foroushani
Volumetric Imaging Using The Pupil-Matched Remote Focusing Technique In Light-Sheet Microscopy, Sayed Hassan Dibaji Foroushani
Optical Science and Engineering ETDs
ABSTRACT
The dissertation explores innovative techniques in light sheet microscopy, a pivotal tool in biomedical imaging, to enhance its speed, resolution, and efficiency in capturing dynamic biological processes. Light sheet microscopy allows for quick 3D imaging of biological specimens ranging from cells to organs with high spatiotemporal resolution, large field-of-view, and minimal damage, making it vital for in vivo imaging.
The first project introduces a novel optical concept designed to optimize Axially Swept Light Sheet Microscopy (ASLM). This technique is crucial for imaging specimens ranging from live cells to chemically cleared organs due to its versatility across different immersion media. …