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Characterization Of Electrophorectic Separations On A Cellulose Paper-Microfluidic Chip, Kyle Robert Fast
Characterization Of Electrophorectic Separations On A Cellulose Paper-Microfluidic Chip, Kyle Robert Fast
Master's Theses
The purpose of this thesis project is to demonstrate the ability to utilize electrophoresis in a cellulose paper microfluidic chip to manipulate charged particles. Materials were selected and a manufacturing protocol was created to successfully apply the electric field onto the paper chip. Experiments were performed to characterize the separation rates for charged, colorimetric dye, Orange G in the membrane as a function of an applied electric field, dye concentration, and distance traveled. The experiments confirmed that the electric field can be applied to the chip and particle separation rates were characterized. Next, the determined rates results were used to …
Characterization Of Pressure-Driven And Electro-Kinetically Driven Flow In A Micro-Fluidic Chip Using Particle Imaging Velocimetry, Alexis Weckel
Characterization Of Pressure-Driven And Electro-Kinetically Driven Flow In A Micro-Fluidic Chip Using Particle Imaging Velocimetry, Alexis Weckel
Master's Theses
The flow profiles of pressure-driven and electro-kinetic driven flows were compared for a microfluidic chip. It was found that the pressure-driven flow had a parabolic profile while the electro-kinetic flow had a plug shaped flow profile. The measured velocities were similar to those determined by the Poiseuille flow model and the Helmholtz-Smoltchowski equation. Flow uniformity is very important for control in microfluidic mixers. Parabolic flow profiles lead to inconsistent reactions while the more uniform plug shape flow allow for a more steady reaction across the channel. Previous work had been performed to measure the flow of a solution of fluorescent …