Engineering Commons^™

Minimizing Wash Water Usage After Acid Hydrolysis Pretreatment Of Biomass, Noaa Frederick

Biological and Agricultural Engineering Undergraduate Honors Theses

Abstract Dilute acid pretreatment, needed to prepare biomass for saccharification, results in the production of a number of byproducts, which inhibit subsequent enzymatic hydrolysis and fermentation steps. In order to improve saccharification yields in the enzyme hydrolysis step, the pretreated biomass is often rinsed with room temperature water to remove these byproducts. High-density poplar was pretreated with 1% dilute sulfuric acid at 140 ºC for 40 minutes. After pretreatment the biomass was washed with water volumes equal to 0, 1 ½, or 3 times the biomass volume. The rinsed biomass was then enzymatically hydrolyzed and the concentrations of byproducts and …