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Impedance Sensing Of N2a And Astrocytes As Grounds For A Central Nervous System Cancer Diagnostic Device, Fraser Traves Smith Grove
Impedance Sensing Of N2a And Astrocytes As Grounds For A Central Nervous System Cancer Diagnostic Device, Fraser Traves Smith Grove
Master's Theses
This thesis utilizes previously described manufacturing and design techniques for the creation of a PDMS-glass bonded microfluidic device, capable of electrochemical impedance spectroscopy (EIS). EIS has been used across various fields of research for different diagnostic needs. The major aim of this thesis was to capture cancerous and non-cancerous cells between micron sized electrodes within a microfluidic path, and to complete analysis on the measured impedances recorded from the two differing cell types. Two distinct ranges of impedance frequency were analyzed – the α dispersion range, which quantifies the impedance of the membranes of the cells of interest, and the …
Quantitative, Spatial Imaging Based Measurements To Assess Cellular Health And Oxygenation In A Tissue Engineered Test System, Erik Bland
All Dissertations
Three-dimensional in vitro tissue test systems are employed to examine cell behavior, test responses to drugs and vaccines, and answer basic biological questions. These systems are more physiologically relevant than two-dimensional cell cultures, and are more relevant, easier and less expensive to maintain than animal models. However, methods used to measure cell behavior and viability have been developed specifically for two-dimensional cell cultures or animal models, and are often not optimally translated to three-dimensional in vitro test systems. The purpose of this work was to aid in the development of three-dimensional, spatially controlled in vitro test systems, and to develop …