Open Access. Powered by Scholars. Published by Universities.®

Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

Theses/Dissertations

Biomedical Engineering and Bioengineering

Cleveland State University

Polypeptides, Elastin, Viscosity, Polymers

Publication Year

Articles 1 - 2 of 2

Full-Text Articles in Engineering

Characterization Of Elastic-Like Polypeptide Micelles Using Capillary Viscometry, Sumit H. Kambow Jan 2012

Characterization Of Elastic-Like Polypeptide Micelles Using Capillary Viscometry, Sumit H. Kambow

ETD Archive

Elastin-like polypeptides (ELPs) are a part of the family of responsive polymers. These polymers can be made to respond to a wide variety of stimuli, including temperature, pH, salt, concentration, light, and solvent. Elastin-like polypeptides are soluble in water at low temperatures but they become hydrophobic and insoluble above their transition temperature. Elastin-like polypeptides, (GVGVP)40-foldon and (GVGVP)60-foldon, were expressed in bacterial system. These ELPs above their transition temperature, Tt, at low salt (< 45mM) and high pH (> pH 10) assemble into micelles where the hydrophobic tails phase separate in the interior of the micelle as an immiscible coacervate phase. The oligomerization domain termed ...


Characterization Of Elastin-Like Polypeptides Using Viscometry, Hamdan Noman Alanazi Jan 2011

Characterization Of Elastin-Like Polypeptides Using Viscometry, Hamdan Noman Alanazi

ETD Archive

Elastin-like polypeptides (ELPs) are a class of polypeptide polymers that are gaining interest in various potential applications. These polymers are responsive to the changes in their environment by exhibiting conformational changes and aggregation. Monodisperse elastin-like polypeptides, (GVGVP)40, (GVGVP)40-foldon, and (GVGVP)60-foldon are made in bacterial expression system. An Ubbelohde capillary viscometer was set up to characterize the structural changes of these ELPs in phosphate buffered saline (PBS) solution. For the ELP-foldon, the relative viscosity measurements were utilized to calculate the intrinsic viscosities using Kraemer and Huggins equations. The known molecular weights of the ELPs and the experimentally determined ...