Engineering Commons^™

Preconditioning Of Primary Human Endothelial Cells With Inflammatory Mediators Alters The “Set Point” Of The Cell, Youichiro Wada, Hasan H. Otu, Shengqian Wu, Md. Ruhul Abid, Hitomi Okada, Towia Libermann, Tatsuhiko Kodama, Shu-Ching Shih, Takashi Minami, William C. Aird

Department of Electrical and Computer Engineering: Faculty Publications

Endothelial cells are highly sensitive to changes in the extracellular milieu. Sepsis results in activation of inflammatory and coagulation pathways. We hypothesized that sepsis-associated mediators may alter the response capacity (so-called “set point”) of endothelial cells. Human umbilical vein endothelial cells (HUVEC) were preincubated in the presence or absence of tumor necrosis factor (TNF)-α, lipopolysaccharide (LPS), hypoxia, hyperthermia, and/or high glucose; treated with or without thrombin for 4 h; and then processed for RNase protection assays of selected activation markers. Priming with TNF-α and LPS significantly inhibited thrombin-mediated induction of vascular cell adhesion molecule-1, intercellular adhesion molecule-1, tissue factor, and …