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New Insights Into Anhydrobiosis Using Cellular Dielectrophoresis-Based Characterization, Mohamed Z. Rashed, Clinton J. Belott, Brett R. Janis, Michael Menze, Stuart J. Williams
New Insights Into Anhydrobiosis Using Cellular Dielectrophoresis-Based Characterization, Mohamed Z. Rashed, Clinton J. Belott, Brett R. Janis, Michael Menze, Stuart J. Williams
Faculty Scholarship
Late embryogenesis abundant (LEA) proteins are found in desiccation-tolerant species from all domains of life. Despite several decades of investigation, the molecular mechanisms by which LEA proteins confer desiccation tolerance are still unclear. In this study, dielectrophoresis (DEP) was used to determine the electrical properties of Drosophila melanogaster (Kc167) cells ectopically expressing LEA proteins from the anhydrobiotic brine shrimp, Artemia franciscana. Dielectrophoresis-based characterization data demonstrate that the expression of two different LEA proteins, AfrLEA3m and AfrLEA6, increases cytoplasmic conductivity of Kc167 cells to a similar extent above control values. The impact on cytoplasmic conductivity was surprising, given …