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Full-Text Articles in Engineering
Cost-Effective Paper-Based Diagnostic Using Split Proteins To Detect Yeast Infections, Zachary R. Berglund, Kevin V. Solomon, Mohit S. Verma, Moiz Rasheed, Zachary Hartley, Kevin Fitzgerald, Kok Zhi Lee, Janice Chan, Julianne Dejoie, Makayla Schacht, Alex Zavala
Cost-Effective Paper-Based Diagnostic Using Split Proteins To Detect Yeast Infections, Zachary R. Berglund, Kevin V. Solomon, Mohit S. Verma, Moiz Rasheed, Zachary Hartley, Kevin Fitzgerald, Kok Zhi Lee, Janice Chan, Julianne Dejoie, Makayla Schacht, Alex Zavala
The Summer Undergraduate Research Fellowship (SURF) Symposium
The common yeast infection, vulvovaginal candidiasis, affects three out of four women throughout their lifetime and can be spread to their child in the form of oral candidiasis (thrush). This disease is caused by the fungal pathogen Candida albicans, which is also a major cause of systemic candidiasis, a rarer but deadly disease with up to a 49% lethality rate. Current widely-used diagnostic methods include cell cultures, pH tests, and antibody detection, to assist effective treatment. Despite availability of various diagnostic methods, there is no inexpensive, rapid, and accurate way to detect C. albicans infection. This project aims to …
Metabolic Comparison Of Wild-Type And Transgenic Synechocystis Pcc 6803 Cyanobacteria, Ian A. Mcluckey, John A. Morgan, Joel Yu King Hing
Metabolic Comparison Of Wild-Type And Transgenic Synechocystis Pcc 6803 Cyanobacteria, Ian A. Mcluckey, John A. Morgan, Joel Yu King Hing
The Summer Undergraduate Research Fellowship (SURF) Symposium
The Calvin-Benson (CBB) cycle is an essential part of nature. This phenomenon allows carbon molecules in carbon dioxide from the atmosphere to be converted into useful energy in the form of sugars. Cyanobacteria are single-celled organisms capable of utilizing energy from sunlight to drive this cycle and are also readily engineered. In hopes of improving this cycle, we compared a wild-type version of the Synechocystis PCC6803 cyanobacteria to an engineered version overexpressing the enzyme FBA (fructose-biphosphate aldolase), called 70 glpX, to deduce how the overexpressing strain is able to be more photosynthetically efficient. To do this, comparative metabolomics were done …
Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus
Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Benzene, a toxin and carcinogen found in air polluted by cigarette smoke, car exhaust, and industrial processes, is associated with the development of leukemia and lymphoma. Other than avoiding exposure, there is no current method to deter the effects of benzene. One potential strategy to prevent these effects is to engineer the bacteria of the human lung microbiome to degrade benzene. To evaluate this novel approach, we must verify that the bacteria remain viable within the lung microenvironment. To do so, lungs were harvested from rats and swabbed to determine the contents of the original lung microbiome. Then green fluorescent …
Inkjet Printing Of Polarized Yeast Cells, Xiuyuan Yang, Kari Clase
Inkjet Printing Of Polarized Yeast Cells, Xiuyuan Yang, Kari Clase
The Summer Undergraduate Research Fellowship (SURF) Symposium
The motivation is to applying engineering knowledge to develop 3D bio-printing in inkjet printer (first stage--monolayer). To achieve the goal, there are three problems to solve. First, we have to figure out regulation of growth of target cells; inability to regulate the location and pattern of growing cells make us even unable to build 3D printer in the direct way. Second problem is how to protect of yeast cells from high temperature and viscous force when printing. The third issue is how to modify the inkjet printer especially the feeding system in order to implement printing on other materials rather …