Engineering Commons^™

Biodegradation Of Phenol And 4-Chlorophenol Using A Single Species In A Sequencing Batch Reactor, Kung-Wei Wang

Theses

Biodegradation of phenol and 4-chlorophenol (4CP) using Pseudomonas putida (ATCC 17514) was studied in batch and sequencing batch reactors. Batch experiments were first performed in order to reveal the kinetics of biodegradation for each substrate. 4-Chlorophenol was degradable only in the presence of phenol, or immediately after exposure of the organism to phenol. It was postulated that an enzyme induced by the presence of phenol was necessary to initiate degradation of 4-chlorophenol.

A mathematical model was then developed to describe the dynamic behavior of both substrates in a sequencing batch reactor (SBR). The model utilized rate parameters obtained from batch …

Reactor Design For Hazardous Waste Treatment Using Immobilized Phanerochaete Chrysosporium, Ikram Ul Haq

Theses

The degradation of 2-Chlorophenol by Phanerochaete chrysosporium, a white rot fungus, was studied in three different reactor configurations, namely a batch reactor, a chemostatic reactor with the fungus immobilized on a silica based porous biocatalyst, and a packed bed reactor utilizing balsa wood chips as packing.

Preliminary experiments indicated that the use of porous solid support improved the degradation rate in batch reactor and chemostatic reactor. However, the packed bed configuration was found to be superior, and was studied in greater detail. The apparent Michaelis-Menten kinetic constants were obtained for the packed bed reactor.

Biodegradation Of Mixed Phenolic Substrates, Prasad Gonnabathula

Theses

The biological degradation of phenol, with either 2-chlorophenol, 2,6-dichlorophenol, or nitrobenzene as cosubstrates was studied in aerated five - liter batch reactors using mixed liquor from the Passaic Valley Sewarage Commissioners wastewater treatment plant (Newark, NJ). The initial substrate concentrations were: 100 – 150 ppm phenol, 10 - 20 ppm 2-cp, 10 ppm 2,3 dcp, and 10 - 20 ppm nitrobenzene. The concentration vs. time data were fit to three kinetic models: zero-order, first-order, and Monod(assuming constant biomass). Most of the data were best fit by either the Monod or zero-order model. For all compounds tested, biodegradation was the primary …

Biodegradation Of Phenol And 2-Chlorophenol Using A Fill-And-Draw-Reactor, Chi-Chun Tsai

Theses

The biological degradation of phenol and 2-chlorophenol was studied at room temperature in a microprocesser controlled fill-and-draw reactor using activated sludge from the Passaic Valley Sewerage Commissioners wastewater treatment plant (Newark, New Jersey). The reactor was cycled through four unit processes: fill, react, settle, and draw. Different cycle times were tested, and the system response was characterized by dissolved oxygen measurements and substrate analysis (by gas chromatography).

With inhibitory substrates, such as those tested, this reactor had much more flexibility, and was therefore much easier to operate, than a standard continuous flow reactor.

The Biodegradation Of Phenolics Using Mixed Liquor From Passaic Valley Sewerage Commissioners Plant, Newark, New Jersey, Daewon Pak

Theses

The biological degradation of phenol, O-chlorophenol, and 2,6-dichlorophenol was studied at room temperature in aerated 5 liter batch reactors using mixed liquor from Passaic Valley Sewerage Commissioners Plant (Newark, New Jersey). From the concentration versus time data, kinetic rate constants were determined for phenol (at 100 ppm), 2'-chlorophenol (at 20 ppm), and 2,6-dichlorophenol (at 10 ppm). Air stripping and adsorption were determined to be insignificant removal mechanisms for the three compounds studied.

It was noted that the biodegradation rates increased after the organisms were acclimated to 100 ppm phenol. In addition, on substrate exposure, the degradation rate increased from the …

A Comparison Of The Biodegradation Of Phenol And O-Chlorophenol Using A Municipal Mixed Liquor And Three Commercial Microbial Preparations, Nigel P. Mcmullen

Theses

The biodegradation of phenol and O-chlorophenol was studied in six-liter batch reactors, using a municipal mixed liquor (from the Livingston, NJ treatment plant) that had not previously been exposed to either of the substrates. In addition, three commercial microbial preparations: BI-CHEM (Sybron), Hydrobac (Polybac), and LLMO (General Environmental Science), were tested alone and in combination with the municipal mixed liquor.

It was found that the municipal mixed liquor performed significantly better than any of the commercial preparations by themselves. When the commercial preparations were mixed with the municipal mixed liquor in a ratio of 1:10 it was found that the …

The Biodegradation Of Phenol And O-Chlorophenol Using Activated Sludge Bacteria, Jeffery Colish

Theses

The biological degradation of phenol up to 500 ppm and 0-chlorophenol up to 40 ppm was studied in an aerated 4.0 liter hatch reactor using activated sludge bacteria from the Livingston, N.J. wastewater treatment plant. From the concentration versus time data, kinetic rate constants were determined for phenol (@ 100 ppm) and o-chlorophenol (@ 20 ppm and 40 ppm). Air stripping was determined to be an insignificant removal mechanism for the compounds studied.

It was noted that the acclimation times decreased with repeated exposure to a particular concentration of phenol or o-chlorophenol, and that the activated sludge bacteria first had …

Kinetics Of Biodegradation Of Phenol And 2,6-Dichlorophenol, Samir Shrikant Desai

Theses

Biological removal of phenol up to 600 ppm and 2,6-dichlorophenol up to 20 ppm was studied in a well aerated fill-and-draw reactor of 41 capacity at 26 C using an activated sludge obtained from the municipal treatment plant in Livingston, N.J. Experimental evaluation of kinetic parameters showed that biodegradation of both phenol and 2,6-DCP followed zero-order kinetics. As expected, the lag time for both compounds was considerably reduced when acclimated sludge was used. However, the metabolism of 2,6-DCP was very slow even for preacclimated sludge, requiring about 36 hours to degrade only 15 ppm.. This was markedly improved by the …