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Full-Text Articles in Engineering
Longitudinal Acoustic Traps: Design, Fabrication, And Evaluation For Biological Applications, Michael Moore Binkley
Longitudinal Acoustic Traps: Design, Fabrication, And Evaluation For Biological Applications, Michael Moore Binkley
McKelvey School of Engineering Theses & Dissertations
Acoustofluidics combine ultrasonic actuation with small-volume microfluidic channels to enable precise, contactless object manipulation for a range of applications from serial chemical processing to blood component separation and single-cell analysis. Micron- to millimeter-scale vibrational waves generate reproducible pressure fields within the microfluidic channels and chambers. By exploiting the material property mismatch between a particle (polymeric and silica beads, cells, etc.) and a suspending fluid, the acoustic radiation force is used to move particles toward regions of low (nodes) or high pressure (antinodes). An understanding of these field-particle interactions is applied to design and implement complicated channel architectures for preferential segregation …
On The Fabrication And Development Of Tissue On A Chip Devices With Included Perfused Vasculatures, Usama Ismail
On The Fabrication And Development Of Tissue On A Chip Devices With Included Perfused Vasculatures, Usama Ismail
McKelvey School of Engineering Theses & Dissertations
The advent of Tissue-on-a-Chip (TiOC) devices has provided a novel way for researchers to approach biological study and drug development. As a platform that enables human in vivo conditions to be accurately replicated ex vivo, TiOC can accelerate both fundamental biological research to answer basic questions regarding tissue behavior and function (e.g., cell-cell interactions in the tumor microenvironment (TME)) and translational research that includes testing of standard and novel therapeutics. The reported work focuses on development of processes and technologies common to two classes of TiOC. The first device is a single-layer, multi-compartment microfluidic device for investigation of pancreatic …
Microfluidic Assessment Of Stem Cell-Derived Pancreatic Beta Cells, Arvind Rajamani Srivatsava
Microfluidic Assessment Of Stem Cell-Derived Pancreatic Beta Cells, Arvind Rajamani Srivatsava
McKelvey School of Engineering Theses & Dissertations
Contemporary and traditional methods of perifusional analysis of cells, pancreatic β cells in particular, involve the utilization of costly equipment exhibiting difficulties in setup and inflexibilities in operation and application. Microfluidic devices in general are inexpensive, easy to fabricate and are capable of screening multiple microenvironmental conditions, with the potential to generate concentration gradients for the study of biological phenomena such as cell migration. Utilization of these devices for cellular fluorescence imaging is also possible, paving the way for more sophisticated methods of dynamic assessment. Herein, we fabricated a pre-designed microfluidic device from photolithography, containing microwells capable of immobilizing human …