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Single Cell Impedance Measurements Using Microfabricated Electrodes And Labview Graphical Programming, Stephanie Sophia Hernandez
Single Cell Impedance Measurements Using Microfabricated Electrodes And Labview Graphical Programming, Stephanie Sophia Hernandez
Master's Theses
This Master’s Thesis project consists of the research, design, and fabrication of a system that could perform broadband impedance measurements (1kHz-20Mhz) of single cells using National Instruments Labview data acquisition and programming in coordination with a single cell capture device. Presented first is the background information on cells and their electrical properties, along with background in micro-total-analysis systems as well as impedance spectroscopy. Experimental Methods are then discussed for the electrode design, cellular modeling in COMSOL, fabrication methods, and Labview 8.0 Set-up and programming. Measurements were performed using the single-cell capture device on saline, yeast cells, and a polysterene bead. …