Engineering Commons^™

The Cooperative Effects Of Channel Length-Bias, Width Asymmetry, Gradient Steepness, And Contact-Guidance On Fibroblasts’ Directional Decision Making, Quang Long Pham

Dissertations

Cell migration in complex micro-environments, that are similar to tissue pores, is important for predicting locations of tissue nucleation and optimizing scaffold architectures. Firstly, how fibroblast cells - relevant to tissue engineering, affect each other’s directional decisions when encountered with a bifurcation of different channel lengths was investigated. It was found that cell sequence and cell mitosis influence the directional choices that the cells made while chemotaxing. Specifically, the fibroblasts chose to alternate between two possible paths - one longer and the other shorter - at a bifurcation. This finding was counter-intuitive given that the shorter path had a steeper …

Microfluidic Biosensor With Functionalized Gold Nano Particles On Interdigitated Electrodes, Bharath Babu Nunna

Dissertations

The integration of the microfluidics to the biosensor has growing demand with favorable conditions such as reduced processing time and low reagent consumption. The immuno biosensing with the microfluidic platform helped to make the electrochemical biosensing assays portable due to which this sensing mechanism can be easily implemented in point of care devices. The implementation of the biosensing in the microchannels significantly reduces the sample requirement form milli liter (mL) to micro liter (uL), and thus leads to low volume sample requirement during the sensing. The primary factors contributing to the microfluidic biosensors performance are probe immobilization, specific binding and …

Electric-Field Assisted Manipulation And Self-Assembly Of Particle Suspensions, Edison Chijioke Amah

Dissertations

The aim of this dissertation is to model the processes by which particles suspended in liquids and at liquid surfaces self-assemble when they are subjected to uniform and non-uniform electric fields. To understand the role of electric forces, three related problems were studied numerically and experimentally.

In the first problem, particles are assumed to be suspended inside a liquid and a nonuniform electric field is applied using electrodes mounted in the domain walls which causes positively polarized particles to collect in the regions where the electric field intensity is locally maximal and the negatively polarized particles collect in the regions …