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Systems Metabolic Engineering Of Microbial Cell Factories For The Synthesis Of Value-Added Chemicals, Arul Mozhy Varman

All Theses and Dissertations (ETDs)

Microbial cell factories offer us an excellent opportunity for the conversion of many different cheaply available raw materials into valuable chemicals. Systems metabolic engineering aims at developing rational strategies for the engineering of microbial hosts by providing global level information of a cell. This dissertation focuses on metabolic engineering, bioprocess modeling and pathway analysis, to develop robust microbial cell factories for the synthesis of value-added chemicals. The following research tasks were completed in this regard.

First, statistical models were developed for the prediction of product yields in engineered microbial cell factories - Saccharomyces cerevisiae and Escherichia coli: Chapter 2). A …