Engineering Commons^™

Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are highly valuable with potent applications, their production at scale remains an outstanding challenge. These molecules are largely produced in Chinese Hamster Ovary (CHO) cells that require highly specific conditions to produce a useful product.

Genetic engineering presents one solution to overcome productivity limits. With the advent of CRISPR (clustered regularly interspaced …

Numerical Modeling And Simulating Thermal Performance Of Printed Circuit Boards, Paul R. Ziegenfelder

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research investigates how heat transfer theory can be applied to evaluate the thermal performance of printed circuit board (PCB) designs. A model was developed for correlating theory to the physical parameters relating to the construction of the PCB and a computer application was developed to perform the thermal analysis. The application allows the importation of common PCB design files for a straightforward and quick analysis of a PCB design’s thermal performance. The application is open-source to allows for further development and free access from the public.

Investigations Of Prokaryotic Defense Systems, Hannah Domgaard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bacteria are constantly threatened with infection by mobile genetic elements (MGE) such as bacteriophage and plasmids. Bacteriophage and plasmids require the bacteria's cellular infrastructure to replicate their genomes. Rampant replication can lead to cell death which is one reason why bacteria have developed a diverse array of immune systems to prevent or limit infection. This thesis studies three types of bacterial immune systems, type IV-A CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeat –CRISPR associated), type V-A2 CRISPR-Cas systems, and Wadjet systems.

The type IV-A system lies adjacent to a dinG-like helicase gene. Research has shown that this system can target …

A Theoretical Trade-Off Between Wave Drag And Sonic Boom Loudness Due To Equivalent Area Changes On A Supersonic Body, Nolan L. Dixon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The NASA University Leadership Initiative (ULI) titled ”Adaptive Aerostructures for Revolutionary Civil Supersonic Transportation” consists of a team of university and industry partners studying the feasibility of reducing the perceived loudness of the sonic boom by introducing an adaptive geometry at localized regions of an aircraft’s outer-mold line. The Utah State University AeroLab is a member of this ULI team and has produced low-fidelity tools to predict the aerodynamic and boom loudness effects from localized changes to the geometry.

Such changes to the geometry affect both the sonic boom loudness and wave drag; however, the precise relationship between boom loudness …

Characterizing Karst Mountain Watersheds Through Streamflow Response To Snowmelt, Daniel Meade Thurber

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The climate in many parts of the Western US is characterized by cold, wet winters preceding long, dry summers. In the absence of precipitation, water supplies in these regions are sustained by melting snow and mountain groundwater. Changes in regional climate can reduce snow accumulation, accelerate melt, and prolong dry periods, all increasing the importance of groundwater on summertime water availability. In mountainous regions with limestone and dolomite geology, bedrock formations can host significant karst aquifers comprising dissolution-enhanced karst conduits which play an outsized and variable role in how precipitation is translated into streamflow. In this study, we considered an …

Design Of Environment Aware Planning Heuristics For Complex Navigation Objectives, Carter D. Bailey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A heuristic is the simplified approximations that helps guide a planner in deducing the best way to move forward. Heuristics are valued in many modern AI algorithms and decision-making architectures due to their ability to drastically reduce computation time. Particularly in robotics, path planning heuristics are widely leveraged to aid in navigation and exploration. As the robotic platform explores and navigates, information about the world can and should be used to augment and update the heuristic to guide solutions. Complex heuristics that can account for environmental factors, robot capabilities, and desired actions provide optimal results with little wasted exploration, but …

Comparison Of Crop Water Use Estimation Methodologies In Irrigated Crops, Laura Christiansen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

As increasing drought events limit water resources available for irrigation, farmers and other water users are looking for ways to monitor how much water crops use over a growing season. The amount of water used by crops over time is the evapotranspiration (ET) rate. This study compares different methods for ET estimation to recommend methods to water users based on their accuracy, efficiency, and accessibility. Each method was used to estimate ET for sprinkler-irrigated corn and alfalfa fields in Modena, UT over the 2021 growing season. The Soil Moisture based ET (SMET) method was used to estimate ET based on …

Event-Based Obstacle Detection With Commercial Lidar, Chaz Cornwall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Computerized obstacle detection for moving vehicles is becoming more important as vehicle manufacturers make their systems more autonomous and safe. However, obstacle detection must operate quickly in dynamic environments such as driving at highway speeds. A unique obstacle detection system using 3D changes in the environment is proposed. Furthermore, these 3D changes are shown to contain sufficient information for avoiding obstacles. To make the system easy to integrate onto a vehicle, additional processing is implemented to remove unnecessary dependencies. This system provides a method for obstacle detection that breaks away from typical systems to be more efficient.

Modeling And Control Of Battery Management Systems With High-Frequency Ac Link Coupled Multiport Series Resonant Converters For 2nd Life Battery Applications, Brooks Jace Maughan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

While the use and production of Electric Vehicles becomes more prevalent, it is also important to make this economical and ensure the reduction of a carbon footprint. Second-life batteries can satisfy both problems as batteries can be used in a second-life application for lower power purposes such as supplementing the grid so the infrastructure needed to charge the expanding fleet of Electric Vehicles can be easily supplied. This thesis goes through the process of Active Cell Balancing which will produce equal capacities, or similar batteries, that can be more efficiently used in these and other types of second-life applications. The …

Lifting-Line Predictions For Life And Twist Distributions To Minimize Induced Drag In Ground Effect, Kyler Church

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The elliptic lift distribution produces the minimum induced drag for a given wingspan and desired lift outside of ground effect. This distribution can be generated on any wing by using geometric and/or aerodynamic twist. However, in ground effect, the elliptic lift distribution is not necessarily that which minimizes induced drag. The present work uses a modern numerical lifting-line algorithm to evaluate how the optimum lift distribution varies as a function of height above ground. The algorithm is also used to obtain the twist distributions that should be applied to wings of varying aspect ratios and taper ratios to produce the …

Linearized Rigid-Body Static And Dynamic Stability Of An Aircraft With A Bio-Inspired Rotating Empennage, Austin J. Kohler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The United States Air Force (USAF) will likely seek to remove the vertical tail of next-generation fighter aircraft. This work seeks to characterize the static and dynamic stability and handling qualities of a vertical-tailless aircraft concept that would satisfy the USAF’s goal. This concept aircraft, one modified with a Bio-Inspired Rotating Empennage (BIRE), does not have a vertical tail, and is instead capable of rotating the horizontal tail about the fuselage axis for maneuvering. The dynamic characteristics of the BIRE-modified aircraft are compared to a baseline unmodified aircraft, similar to the F16, with a traditional vertical tail. Linearized aerodynamic models …

Developing A Methane Detector For Aerospace Applications, Michael A. Kirk

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greenhouse gasses in the atmosphere are raising the global temperature and causing adverse side effects. Of these greenhouse gasses, methane is one of the most impactful, second only to carbon dioxide. One of the methods for determining the concentration of methane in the atmosphere is taking images of the earth from space. The purpose of this project is to further a new imaging technology for detecting methane leaks called FINIS (Filter Incidence Narrow-band Infrared Spectrometer), thus improving our capability to detect and locate methane leaks and reduce greenhouse gas emissions. FINIS has been developed in various stages since 2018 and …

Low Resource Assay For Tracking Sars-Cov-2 In Wastewater, Julissa Van Renselaar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wastewater based epidemiology (WBE) allows for the tracking of nucleic acid of SARS-CoV-2 in wastewater which gives the opportunity for the public and government officials to be informed about the infectivity of the virus in a community. Advances have been made in WBE that have allowed for higher performance, lower resource use, and faster turnaround time. An adapted concentration method of spin column direct extraction has yielded a proxy virus recovery of 83%, consumable cost of $2.01 per sample, and a turnaround time of 0.33 hour/sample. Other concentration methods have lower proxy virus recoveries, comparable cost, and comparable turnaround time. …

Formative Assessment In Engineering Education: Exploring Ways To Enhance Students' Learning Achievement, Assad Iqbal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Formative assessments have been found to enhance students’ learning across a variety of disciplines, educational levels, and laboratory and classroom settings. Research attributes the positive effects of formative assessments in improving students’ learning to its (backward and forward) testing effects. Moreover, formative assessments provide an extra opportunity to students to assess their learning early in the learning process, reflect on their learning, and identify address learning gaps and misconceptions (if any) using feedback.

However, optional nature of formative assessments and having no stakes associated with them to have any bearing on final grades offers two challenges to capitalize on their …

Multi-Fidelity Predictions For Control Allocation On The Nasa Ikhana Research Aircraft To Minimize Drag, Justice T. Schoenfeld

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Optimal control settings (camber scheduling) can be used by aircraft to minimize drag at various operating conditions during flight. In this work, camber schedules for minimum drag on the NASA Ikhana are obtained over a range of lift coefficients. A modern numerical lifting-line algorithm is used to predict the lift and drag of the aircraft as a function of operating condition and wing section shape (airfoil camber). The SLSQP optimization algorithm is used to solve for the camber schedule that minimizes drag for a given operating condition. The process is repeated, varying the number of control sections to evaluate the …

Path Planning For Aircraft Under Threat Of Detection From Ground-Based Radar With Uncertainty In Aircraft And Radar States, Austin D. Costley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mission planners for manned and unmanned aircraft operating within the detection range of ground-based radar systems are often concerned with the probability of detection. Several factors influence the probability of detection, including aircraft position and orientation, radar position, and radar performance parameters. Current path planning algorithms assume that these factors are known with certainty, but in practice, these factors are estimated and have some uncertainty.

This dissertation explores methods to consider the uncertainty in the detection factors for an aircraft path planner. First, the detection model is extended to include uncertainty in the aircraft position and orientation, radar position, and …

High-Isolation Antenna Technique For Cubesat-Borne, Continuous-Waveform Radar, Logan E. Voigt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Radar is important in target tracking, imaging, and weather prediction applications. As technology is increasingly miniaturized, there is a push for smaller radar. Research and exploration in outer space also benefit from small, low-power technologies. The NASA Jet Propulsion Laboratory’s RainCube was the first successful CubeSat-borne radar. A CubeSat is a type of small satellite that conforms to specific size and weight standards. Radar technology benefits from additional research on how to further miniaturize radar payloads.

Integrating the transmit and receive antennas on the solar panels removes the need for antenna-deployment mechanisms, preserving space on the CubeSat. This thesis also …

Advancing The Cyberinfrastructure For Smart Water Metering And Water Demand Modeling, Nour A. Attallah

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

With rapid growth of urban populations and limited water resources, achieving an appropriate balance between water supply capacity and residential water demand poses a significant challenge to water supplying agencies. With the recent emergence of smart metering technology, where water use can be monitored and recorded at high resolution (e.g., observations of water use every 5 seconds), most existing research has been aimed at providing water managers with detailed information about the water use behavior of their consumers and the performance of water using fixtures. However, replacing existing meters with smart meters is expensive, and effectively using data produced by …

Using Computational Fluid Dynamics To Predict Flow Through The West Crack Breach Of The Great Salt Lake Railroad Causeway, Michael Rasmussen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Great Salt Lake in Utah, USA, is a terminal, saline lake and is divided into two primary sections (northern and southern) by an east-to-west railroad causeway. Shortly after completion of the earth-fill causeway in the late 1950s, the two sections became dramatically different with differences in water surface elevation and water density. These differences cause the formation of a unique flow behavior commonly referred to as a density-driven exchange flow or bi-directional flow; a behavior observed in other lake and ocean settings where two fluids of differing densities interact. Measuring these exchange flows is a priority for lake managers …

Cuo Nanoparticles Solubility As Influenced By Soil Pore Water, Native Microorganisms, And Wheat Rhizosphere Chemistry In A Sand Matrix, Dakota Sparks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nanoparticles (NPs) are particles less than 100 nm (~4 millionths of an inch) in a direction. NPs, due to their small size, are used in a variety of products, such as silver (Ag) NPs as an antimicrobial in clothes. Copper Oxide (CuO) NPs are used in electronics as semiconductors and other fields as antimicrobials and purposefully or accidentally end up in the environment. Copper (Cu) is a necessary nutrient for plants, but at higher amounts is toxic to plants and beneficial soil microbes.

In order to understand how the CuO NPs interacts with plants, wheat seedlings were grown in sand …

Analysis Of Optically Transparent Antennas Designed From Different Transparent Conductors, Rakib Hasan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This master’s thesis work presents an investigative study about the performance of transparent antennas. The antennas have been fabricated using two advanced transparent conductive films (TCF) namely Nano-C Hybrid and Silver-Nanowire (AgNW). Nano-C Hybrid is a new transparent conductive film which combines thin conductors like Carbon Nanotube and Silver Nanowire. The antennas made from Nano-C Hybrid and AgNW are of monopole types and are mounted on a ground plane made out of a printed circuit board and the antennas are excited using an SMA connector through that printed circuit board. Simulation works have been performed to find out the optimum …

Lentiviral Vector Production At High Cell Density By Transient Transfection Of Suspended Culture Hek Cells, Jacob G. Accordino

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Viral vectors are gene carriers that efficiently deliver therapeutic gene constructs to target cells. Viral vectors are frequently produced in suspension-growing cells of mammalian origin, referred to as packaging cells. Lentiviral vectors have become widely used as gene vectors since they were initially developed two and a half decades ago, particularly in research settings. The advantages of lentiviral vectors for treating diseases of genetic origin have driven research into their large-scale manufacture for clinical settings. Currently, three main challenges exist that limit mass production of lentiviral vectors. First, plasmid DNA used in the production process is very expensive. Second, high …

Control Mapping Methodology For Tailless Morphing-Wing Aircraft, Zachary S. Montgomery

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Advanced aircraft designs tend to have several control surfaces or devices that affect the flight of the aircraft. It is difficult or even impossible for a pilot to directly control each of these devices and fly the aircraft well. Therefore, a control mapping logic is needed to take typical pilot commands and map them to what the control devices should do to achieve the pilot’s commands. This work presents a methodology for determining this control mapping logic using two different approaches. The first uses a theoretical approach based on lifting-line theory, while the second leverages computational methods. The methodology consists …

Multidisciplinary Reference Solutions For Performance-Optimized Aircraft Wings With Tailored Aerodynamic Load Distributions, Jeffrey D. Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Morphing wings, or wings that can change shape during flight, have the potential to substantially reduce the amount of fuel consumed by an aircraft over the course of its flight. However, the extent to which these wings can reduce fuel consumption depends on the design of the wing, including its aerodynamic efficiency and its structural layout, and how the aircraft flies, including its flight altitude and speed. Correctly predicting how these design and operational characteristics interact is critical to predicting how wing morphing may affect aircraft fuel consumption. Many computer prediction tools exist that include the effects of these interactions, …

Sustain Water Conservation Behaviors Using Nonparametric Ranking And Social Marketing, Mahmudur Rahman Aveek

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Circulation of water use feedback and conservation messaging are strategies implemented to reduce water demand on a short-term (seasonal) basis. Often considered a less impactful strategy than other tactics such as price increments and usage restrictions, authorities mostly use feedback in informational campaigns with a focus to apprise users about their water use. Such conservation programs have had limited success that has been attributed to the fact that the information provided with the feedback campaigns was generic and did not motivate users enough to sustain their water-saving behaviors. However, the advent of disaggregation technologies that can provide appliance-wise water use …

Bugs Buy Steady Releases From Hydropower Producers To Encourage More Synergistic Reservoir Management, Moazzam Ali Rind

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Hydropower generated from dams has significant economic value, however, that value is achieved at the cost of native ecosystem devastation. Here, we have estimated loss in hydropower revenue due to inclusion of the steady low flow days –Bug Flow Experiments. We developed a linear optimization model and constraint method that restrict the number of steady low flow days while maximizes the hydropower revenue generation. The results suggested that increase in release volume will benefit both the objectives (win-win scenario), energy price differential between on-and off-peak periods controls the position and shape of tradeoff curves, and offset release does not have …

Pedestrian Behavior At Signalized Intersections Throughout Utah, Sadie Boyer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pedestrians and vehicles interact with each other all over the world. Pedestrian-vehicle interactions are most likely to occur at intersections. One way to streamline these interactions and reduce the number of potential conflicts is by using traffic signals. Signalized intersections were developed to increase the overall safety and efficiency of movements involving motorists and, later, pedestrians (Clark, 2022). The number of signalized intersections is increasing across the country as vehicle volumes increase. This means that pedestrian-vehicle conflicts are also increasing. Pedestrian-vehicle conflicts can have serious, even fatal, consequences if not appropriately managed.

This study was a sponsored project by the …

Highly Variable Rainfall-Runoff Patterns Across Burned Mountainous Watersheds In The Colorado River Headwaters, Haley Anne Canham

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wildfires can contribute to enhanced flooding, erosion, debris flows, sediment transport, and water quality changes that impact downstream infrastructure, water users, and aquatic habitat. With increasing wildfire risk in the western U.S. due to a changing climate, understanding post-wildfire rainfall-runoff patterns and controls is critical for continued water resources security. To improve understanding of post-wildfire rainfall-runoff patterns and controls, we developed a transparent, repeatable analysis framework to collect precipitation and streamflow data, identify paired rainfall-runoff events, and analyze these events to evaluate post-wildfire rainfall-runoff patterns and controls. To automate the rainfall-runoff event identification, the Rainfall-Runoff Event Detection and Identification (RREDI) …

Passage Probability Of Woody Debris Elements And Accumulations At I- And V-Shaped Rock Weirs, Kathryn Margetts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The transport and accumulation of driftwood or large woody debris (LWD) in mountain streams is a natural part of catchment health and river connectivity. At hydraulic structures, the presence of LWD may impact flow efficiency and influence upstream water depth. LWD has been studied at a variety of spillways and weir types, however, little is known about its interaction at rock weirs. This study investigated factors affecting the passage of individual LWD elements and any LWD accumulations at rock weirs, as well as potential impacts upstream of LWD accumulations through field-informed scaled model testing. Observations of LWD at rock weirs …

Upstream Methods For Enhancing Engineered Curcumin Biosynthesis, Caleb D. Barton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Curcumin is a bright orange compound with myriad applications for human health and wellness. Curcumin occurs naturally in the plant Curcuma longa (commonly known as turmeric) but must be extracted from the roots in an environmentally unfriendly fashion to obtain commercially relevant amounts of the compound. In addition, extraction of curcumin from turmeric spice yields a mixture of various curcuminoids, presenting an issue for isolating it in its pure form and complicating its use in clinical settings.

Heterologous biosynthetic production of curcumin in Escherichia coli has been used extensively as a viable alternative to plant extraction but suffers from poor …