Engineering Commons^™

Study On The Interactions Between Nanomaterials And Proteins, Yueqi Bi

Electronic Thesis and Dissertation Repository

Incorporating proteins with nanomaterials is an effective way to enhance the stability and function of proteins. The protein-nanomaterials hybrid systems have been extensively applied in drug delivery and biocatalysis. This thesis focuses on the different interactions between proteins and nanomaterials. Three sub-projects have been studied as follows;

1) Chemical interaction: Gold nanoparticles with a particle size of 15nm were applied to label bovine serum albumin (BSA), a globular protein, for realizing a colorimetric protein assay. The results of FTIR and Raman spectra indicate that gold nanoparticles bond to BSA via the amine bonds. The surface plasma resonance (SPR) of …

Encapsulation And Controlled Release Of Rhu-Erythropoietin From Chitosan Biopolymer Nanoparticles, Cody Bulmer

Electronic Thesis and Dissertation Repository

The objective of this research project was to develop a drug delivery system for recombinant human erythropoietin (rHu-EPO), a glycoprotein hormone used in the treatment of renal anaemia and chemotherapy induced anaemia, using the biopolymer chitosan as the base component. Two types of chitosan nanoparticles were produced through ionotropic gelation using flush mixing with either tripolyphosphate (TPP) or carrageenan polymer. Chitosan-TPP and chitosan-carrageenan nanoparticles were generated under a variety of conditions to evaluate the effects of chitosan concentration, chitosan to anion mass ratio and solution pH on the nanoparticle characteristics of particle diameter, surface charge and particle size distribution. A …