Engineering Commons^™

Real-Time Registration And Display Of Confocal Microscope Imagery For Multiple-Band Analysis, Scott E. Budge, A.M. Mayampurath, J.C. Solinsky

Electrical and Computer Engineering Faculty Publications

Multiple images acquired in real-time from a confocal microscope in different illumination wavebands are used to construct a three-dimensional understanding of living cell signal propagation. Registration, fusion, and display of these images are performed in real-time using field programmable gate array (FPGA) technology to allow the biological scientist to observe time critical signaling processes as they occur. The registration and display system operates at a rate of up to 15 frames/s, and performs dark offset correction and noise reduction, followed by non-linear correction for lens warp and focal plane tilt, and finally correction for spatial mismatch between the different wavebands. …