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Full-Text Articles in Engineering
High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Chritopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittus, William N. Drohan
High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Chritopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittus, William N. Drohan
Papers in Biotechnology
Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 pg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from …
In Vitro Development Of Zygotes From Prepubertal Gilts After Microinjection Of Dna, B. L. Williams, J. L. Johnson, A. E.T. Sparks, R. S. Canseco, J. W. Knight, William H. Velander, R. L. Page, W. N. Drohan, J. M. Young, R. E. Pearson, T. D. Wilkins, F. C. Gwazdauskas
In Vitro Development Of Zygotes From Prepubertal Gilts After Microinjection Of Dna, B. L. Williams, J. L. Johnson, A. E.T. Sparks, R. S. Canseco, J. W. Knight, William H. Velander, R. L. Page, W. N. Drohan, J. M. Young, R. E. Pearson, T. D. Wilkins, F. C. Gwazdauskas
Papers in Biotechnology
The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and hCG. Zygotes received either pronuclear microinjection of buffer alone, buffer containing a DNA construct, or no microinjection. Zygotes were cultured in vitro in either modified Krebs-Ringer bicarbonate medium (KRBI for 144 h or in mouse oviduct (MO) explant culture with KRB for 48, 72, 96, or 120 h. Pronuclear microinjection of DNA resulted in a lower (P < .05) cleavage index (CII than did buffer or no microinjection (CI 2.16 k .10 vs 2.80 f .13 and 2.93 f .lo). The CI loss was greatest for DNA-injected zygotes at the two-cell stage of development. Coculture of zygotes in MO resulted in a higher CI (P < .01) than did culture in KRB. Culture in MO for 72 h was the most beneficial system compared with MO for 48, 96, or 120 h (P < .05; CI 3.25 k .12 vs 2.66 k .18, 2.79 f .14, and 2.40 .14, respectively). Microinjection of DNA, not merely the mechanical procedure, was detrimental to early zygote development and may be the cause of low pregnancy rates.