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Identification And Expression Analysis Of Two Inorganic C-And N-Responsive Genes Encoding Novel And Distinct Molecular Forms Of Eukaryotic Phosphoenolpyruvate Carboxylase In The Green Microalga Chlamydomonas Reinhardtii, Tarlan G. Mamedov, Eric R. Moellering, Raymond Chollet
Identification And Expression Analysis Of Two Inorganic C-And N-Responsive Genes Encoding Novel And Distinct Molecular Forms Of Eukaryotic Phosphoenolpyruvate Carboxylase In The Green Microalga Chlamydomonas Reinhardtii, Tarlan G. Mamedov, Eric R. Moellering, Raymond Chollet
Papers in Biochemical Engineering
Phosphoenolpyruvate carboxylase (PEPC [Ppc]) has been previously purified and characterized in biochemical and immunological terms from two green microalgae, Chlamydomonas reinhardtii and Selenastrum minutum. The findings indicate that these algae possess at least two distinct PEPC enzyme-forms, homotetrameric Class1 and heteromeric Class-2 that differ significantly from each other and their plant and prokaryotic counterparts. Surprisingly, however, green-algal PEPC has been unexplored to date in molecular terms. This study reports the molecular cloning of the two Ppc genes in C. reinhardtii (CrPpc1, CrPpc2), each of which is transcribed in vivo and encodes a fully active, recombinant PEPC that lacks the regulatory, …