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Transgenic Mammals Expressing Human Coagulation Factor Viii , William H. Velander
Transgenic Mammals Expressing Human Coagulation Factor Viii , William H. Velander
Department of Chemical and Biomolecular Engineering: Patents
A non-human transgenic mammalian animal, as described above, contains an exogenous double stranded DNA sequence stably integrated into the genome of the animal, which comprises cis-acting regulatory units operably linked to a DNA sequence encoding human Factor VIII protein and a signal peptide, where the cis-acting regulatory units are active in mammary gland cells and the signal peptide is active in directing newly expressed Factor VIII into the milk of the animal. The promoter may be a milk protein promoter such as for whey acidic protein, casein, lactalbumin, or beta-lactoglobulin promoter. The transgenic mammals are preferably farm animals, for example, …
Expression Of Human Protein C In Mammary Tissue Of Transgenic Mammals , William H. Velander
Expression Of Human Protein C In Mammary Tissue Of Transgenic Mammals , William H. Velander
Department of Chemical and Biomolecular Engineering: Patents
Recombinant protein C characterized by a high percentage of active protein can be obtained in the milk of transgenic mammals that incorporate DNAs according to the present invention. Transgenic mammals of the present invention are produced by introducing into developing embryos DNA that encodes protein C, such that the DNA is stably incorporated in the DNA of germ line cells of the mature mammals and inherited in normal, mendelian fashion.