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University of Nebraska - Lincoln

Department of Biological Systems Engineering: Papers and Publications

2016

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Articles 1 - 30 of 44

Full-Text Articles in Engineering

Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen Dec 2016

Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen

Department of Biological Systems Engineering: Papers and Publications

Streambanks can be a significant source of sediment and phosphorus to aquatic ecosystems. Although the streambank-erosion routine in the Soil and Water Assessment Tool (SWAT) has improved in recent versions, the recently developed routine in SWAT 2012 has undergone limited testing, and the lack of site or watershed specific streambank data increases the uncertainty in the streambank-erosion predictions. There were two primary objectives of this research: (1) modify and test the 2012 SWAT streambank-erosion routine on composite streambanks, and (2) compare SWAT default and field-measured channel parameters and assess their influence on predicted streambank erosion. Three modifications were made to …


Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova Nov 2016

Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova

Department of Biological Systems Engineering: Papers and Publications

Novel groundwater sampling (age, flux, and nitrate) carried out beneath a streambed and in wells was used to estimate (1) the current rate of change of nitrate storage, dSNO3 /dt, in a contaminated unconfined aquifer, and (2) future [NO3]FWM (the flow-weighted mean nitrate concentration in groundwater discharge) and fNO3 (the nitrate flux from aquifer to stream). Estimates of dSNO3 /dt suggested that at the time of sampling (2013) the nitrate storage in the aquifer was decreasing at an annual rate (mean = –9 mmol/m2yr) equal …


Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader Nov 2016

Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader

Department of Biological Systems Engineering: Papers and Publications

Runoff generated from livestock manure amended row crop fields is one of the major pathways of hormone transport to the aquatic environment. The study determined the effects of manure handling, tillage methods, and rainfall timing on the occurrence and transport of steroid hormones in runoff from the row crop field. Stockpiled and composted manure from hormone treated and untreated animals were applied to test plots and subjected to two rainfall simulation events 30 days apart. During the two rainfall simulation events, detection of any steroid hormone or metabolites was identified in 8–86% of runoff samples from any tillage and manure …


Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet Sep 2016

Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet

Department of Biological Systems Engineering: Papers and Publications

Wetlands and coastal systems are unique, highly productive, and often threatened landscapes that provide a host of services to both humans and the environment. This article introduces a five-article Wetlands and Coastal Systems Special Collection that evolved from a featured session at the 2015 ASABE Annual International Meeting in New Orleans, Louisiana. The Collection provides perspectives on tools and techniques for enhancing the protection and restoration of wetlands and coastal systems with emphasis on vegetation, hydrology, water quality, and planning. Topics span the Florida Everglades (two articles) and Virginia floodplain (one article) wetland systems and include remote sensing (one article) …


Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang Jul 2016

Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang

Department of Biological Systems Engineering: Papers and Publications

Advances in automation and data science have led agriculturists to seek real-time, high quality, high-volume crop data to accelerate crop improvement through breeding and to optimize agronomic practices. Breeders have recently gained massive data-collection capability in genome sequencing of plants. Faster phenotypic trait data collection and analysis relative to genetic data leads to faster and better selections in crop improvement. Furthermore, faster and higher-resolution crop data collection leads to greater capability for scientists and growers to improve precision-agriculture practices on increasingly larger farms; e.g., site-specific application of water and nutrients. Unmanned aerial vehicles (UAVs) have recently gained traction as agricultural …


High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier Jul 2016

High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Nonviral gene delivery methods are advantageous over viral vectors in terms of safety, cost, and flexibility in design and application, but suffer from lower gene transfer efficiency. In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. Priming is the pharmacological modulation of transfection efficiency and transgene expression, and has demonstrated transfection increase in several compounds, for example, chloroquine and glucocorticoids. To develop a library of transfection priming compounds, a highthroughput screen was performed of the NIH Clinical Collection (NCC) to identify clinical compounds that prime polyethylenimine (PEI) transfection. HEK293T cells were …


Efficiency Of Chlorophyll In Gross Primary Productivity: A Proof Of Concept And Application In Crops, Anatoly A. Gitelson, Yi Peng, Andrés Viña, Timothy J. Arkebauer, James S. Schepers Jun 2016

Efficiency Of Chlorophyll In Gross Primary Productivity: A Proof Of Concept And Application In Crops, Anatoly A. Gitelson, Yi Peng, Andrés Viña, Timothy J. Arkebauer, James S. Schepers

Department of Biological Systems Engineering: Papers and Publications

One of the main factors affecting vegetation productivity is absorbed light, which is largely governed by chlorophyll. In this paper, we introduce the concept of chlorophyll efficiency, representing the amount of gross primary production per unit of canopy chlorophyll content (Chl) and incident PAR. We analyzed chlorophyll efficiency in two contrasting crops (soybean and maize). Given that they have different photosynthetic pathways (C3 vs. C4), leaf structures (dicot vs. monocot) and canopy architectures (a heliotrophic leaf angle distribution vs. a spherical leaf angle distribution), they cover a large spectrum of biophysical conditions. Our results show that chlorophyll efficiency in primary …


Micro- And Nanoparticulates For Dna Vaccine Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier Apr 2016

Micro- And Nanoparticulates For Dna Vaccine Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

DNA vaccination has emerged as a promising alternative to traditional protein-based vaccines for the induction of protective immune responses. DNA vaccines offer several advantages over traditional vaccines, including increased stability, rapid and inexpensive production, and flexibility to produce vaccines for a wide variety of infectious diseases. However, the immunogenicity of DNA vaccines delivered as naked plasmid DNA is often weak due to degradation of the DNA by nucleases and inefficient delivery to immune cells. Therefore, biomaterial-based delivery systems based on micro- and nanoparticles that encapsulate plasmid DNA represent the most promising strategy for DNA vaccine delivery. Microparticulate delivery systems allow …


Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Suat Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin Feb 2016

Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Suat Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin

Department of Biological Systems Engineering: Papers and Publications

Evapotranspiration (ET) processes at the leaf to landscape scales in multiple land uses have important controls and feed backs for local, regional, and global climate and water resource systems. Innovative methods, tools, and technologies for improved understanding and quantification of ET and crop water use are critical for adapting more effective management strategies to cope with increasing demand for freshwater resources under global climate change. This article introduces an ASABE Special Collection of 12 articles on ET monitoring and modeling research for multiple land uses and scales. The collection focuses on recent advances in four critical topical areas: (1) reference …


Glucocorticoid Cell Priming Enhances Transfection Outcomes In Adult Human Mesenchymal Stem Cells, Abby M. Kelly, Sarah A. Plautz, Janos Zempleni, Angela K. Pannier Feb 2016

Glucocorticoid Cell Priming Enhances Transfection Outcomes In Adult Human Mesenchymal Stem Cells, Abby M. Kelly, Sarah A. Plautz, Janos Zempleni, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Human mesenchymal stem cells (hMSCs) are one of the most widely researched stem cell types with broad applications from basic research to therapeutics, the majority of which require introduction of exogenous DNA. However, safety and scalability issues hinder viral delivery, while poor efficiency hinders nonviral gene delivery, particularly to hMSCs. Here, we present the use of a pharmacologic agent (glucocorticoid) to overcome barriers to hMSC DNA transfer to enhance transfection using three common nonviral vectors. Glucocorticoid priming significantly enhances transfection in hMSCs, demonstrated by a 3-fold increase in efficiency, 4–15-fold increase in transgene expression, and prolonged transgene expression when compared …


In-Field Fuel Use And Load States Of Agricultural Field Machinery, Santosh Pitla, Joe D. Luck, Jared Werner, Nannan Lin, Scott A. Shearer Jan 2016

In-Field Fuel Use And Load States Of Agricultural Field Machinery, Santosh Pitla, Joe D. Luck, Jared Werner, Nannan Lin, Scott A. Shearer

Department of Biological Systems Engineering: Papers and Publications

The ability to define in-field tractor load states offers the potential to better specify and characterize fuel consumption rate for various field operations. For the same field operation, the tractor experiences diverse load demands and corresponding fuel use rates as it maneuvers through straight passes, turns, suspended operation for adjustments, repair and maintenance, and biomass or other material transfer operations. It is challenging to determine the actual fuel rate and load states of agricultural machinery using force prediction models, and hence, some form of in-field data acquisition capability is required. Controller Area Networks (CAN) available on the current model tractors …


Direct And Indirect Effects Of Climatic Variations On The Interannual Variability In Net Ecosystem Exchange Across Terrestrial Ecosystems, Junjiong Shao, Xuhui Zhou, Yiqi Luo, Bo Li, Mika Aurela, David Billesbach, Peter D. Blanken, Rosvel Bracho, Jiquan Chen, Marc Fischer, Yuling Fu, Lianhong Gu, Shijie Han, Yongtao He, Thomas Kolb, Yingnian Li, Zoltan Nagy, Shuli Niu, Walter C. Oechel, Krisztina Pinter, Peili Shi, Andrew Suyker, Margaret Torn, Andrej Varlagin, Huimin Wang, Junhua Yan, Guirui Yu, Junhui Zhang Jan 2016

Direct And Indirect Effects Of Climatic Variations On The Interannual Variability In Net Ecosystem Exchange Across Terrestrial Ecosystems, Junjiong Shao, Xuhui Zhou, Yiqi Luo, Bo Li, Mika Aurela, David Billesbach, Peter D. Blanken, Rosvel Bracho, Jiquan Chen, Marc Fischer, Yuling Fu, Lianhong Gu, Shijie Han, Yongtao He, Thomas Kolb, Yingnian Li, Zoltan Nagy, Shuli Niu, Walter C. Oechel, Krisztina Pinter, Peili Shi, Andrew Suyker, Margaret Torn, Andrej Varlagin, Huimin Wang, Junhua Yan, Guirui Yu, Junhui Zhang

Department of Biological Systems Engineering: Papers and Publications

Climatic variables not only directly affect the interannual variability (IAV) in net ecosystem exchange of CO2 (NEE) but also indirectly drive it by changing the physiological parameters. Identifying these direct and indirect paths can reveal the underlying mechanisms of carbon (C) dynamics. In this study, we applied a path analysis using flux data from 65 sites to quantify the direct and indirect climatic effects on IAV in NEE and to evaluate the potential relationships among the climatic variables and physiological parameters that represent physiology and phenology of ecosystems. We found that the maximum photosynthetic rate was the most important factor …


Heterogeneity Influences On Stream Water–Groundwater Interactions In A Gravel-Dominated Floodplain, R. B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm Jan 2016

Heterogeneity Influences On Stream Water–Groundwater Interactions In A Gravel-Dominated Floodplain, R. B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm

Department of Biological Systems Engineering: Papers and Publications

Floodplains are composed of complex depositional patterns of ancient and recent stream sediments, and research is needed to address the manner in which coarse floodplain materials affect stream/groundwater exchange patterns. Efforts to understand the heterogeneity of aquifers have utilized numerous techniques typically focused on point-scale measurements; however, in highly heterogeneous settings, the ability to model heterogeneity is dependent on the data density and spatial distribution. The objective of this research was to investigate the correlation between broad-scale methodologies for detecting heterogeneity and the observed spatial variability in stream/groundwater interactions of gravel-dominated alluvial floodplains. More specifically, this study examined the correlation …


Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Sibel Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin Jan 2016

Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Sibel Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin

Department of Biological Systems Engineering: Papers and Publications

Evapotranspiration (ET) processes at the leaf to landscape scales in multiple land uses have important con- trols and feedbacks for local, regional, and global climate and water resource systems. Innovative methods, tools, and technologies for improved understanding and quantification of ET and crop water use are critical for adapting more effective management strategies to cope with increasing demand for freshwater resources under global climate change. This article introduces an ASABE Special Collection of 12 articles on ET monitoring and modeling research for multiple land uses and scales. The collection focuses on recent advances in four critical topical areas: (1) reference …


Objective Climatological Analysis Of Extreme Weather Events In Arizona During The North American Monsoon, Jeremy J. Mazon, Christopher L. Castro, David K. Adams, Hsin-I Chang, Carlos M. Carrillo, John J. Brost Jan 2016

Objective Climatological Analysis Of Extreme Weather Events In Arizona During The North American Monsoon, Jeremy J. Mazon, Christopher L. Castro, David K. Adams, Hsin-I Chang, Carlos M. Carrillo, John J. Brost

Department of Biological Systems Engineering: Papers and Publications

Almost one-half of the annual precipitation in the southwestern United States occurs during the North American monsoon (NAM). Given favorable synoptic-scale conditions, organized monsoon thunderstorms may affect relatively large geographic areas. Through an objective analysis of atmospheric reanalysis and observational data, the dominant synoptic patterns associated with NAM extreme events are determined for the period from 1993 to 2010. Thermodynamically favorable extreme-weather-event days are selected on the basis of atmospheric instability and precipitable water vapor from Tucson, Arizona, rawinsonde data. The atmospheric circulation patterns at 500 hPa associated with the extreme events are objectively characterized using principal component analysis. The …


Critical Factors Affecting The Integration Of Biomass Gasification And Syngas Fermentation Technology, Karthikeyan D. Ramachandriya, Dimple K. Kundiyana, Ashokkumar M. Sharma, Ajay Kumar, Hasan K. Atiyeh, Raymond L. Huhnke, Mark R. Wilkins Jan 2016

Critical Factors Affecting The Integration Of Biomass Gasification And Syngas Fermentation Technology, Karthikeyan D. Ramachandriya, Dimple K. Kundiyana, Ashokkumar M. Sharma, Ajay Kumar, Hasan K. Atiyeh, Raymond L. Huhnke, Mark R. Wilkins

Department of Biological Systems Engineering: Papers and Publications

Gasification-fermentation is a thermochemical-biological platform for the production of fuels and chemicals. Biomass is gasified at high temperatures to make syngas, a gas composed of CO, CO2, H2, N2 and other minor components. Syngas is then fed to anaerobic microorganisms that convert CO, CO2 and H2 to alcohols by fermentation. This platform offers numerous advantages such as flexibility of feedstock and syngas composition and lower operating temperature and pressure compared to other catalytic syngas conversion processes. In comparison to hydrolysis-fermentation, gasification-fermentation has a major advantage of utilizing all organic components of biomass, including lignin, to yield higher fuel production. Furthermore, …


Long-Term Patterns Of Air Temperatures, Daily Temperature Range, Precipitation, Grass-Reference Evapotranspiration And Aridity Index In The Usa Great Plains: Part I. Spatial Trends, Meetpal S. Kukal, Suat Irmak Jan 2016

Long-Term Patterns Of Air Temperatures, Daily Temperature Range, Precipitation, Grass-Reference Evapotranspiration And Aridity Index In The Usa Great Plains: Part I. Spatial Trends, Meetpal S. Kukal, Suat Irmak

Department of Biological Systems Engineering: Papers and Publications

Due to their substantial spatio-temporal behavior, long-term quantification and analyses of important hydrological variables are essential for practical applications in water resources planning, evaluating the water use of agricultural crop production and quantifying crop evapotranspiration patterns and irrigation management vs. hydrologic balance relationships. Observed data at over 800 sites across the Great Plains of USA, comprising of 9 states and 2,307,410 km2 of surface area, which is about 30% of the terrestrial area of the USA, were used to quantify and map large-scale and long-term (1968-2013) spatial trends of air temperatures, daily temperature range (DTR), precipitation, grass-reference evapotranspiration (ET …


Removal Of Cattle Manure Constituents In Runoff From No-Till Cropland As Affected By Setback Distance, John E. Gilley, Aaron J. Sindelar, Bryan L. Woodbury Jan 2016

Removal Of Cattle Manure Constituents In Runoff From No-Till Cropland As Affected By Setback Distance, John E. Gilley, Aaron J. Sindelar, Bryan L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

Vegetative filter strips located at the bottom of a hillslope have been shown to substantially reduce nutrients and sediment in runoff. Cropland areas could serve a similar function. However, little scientifically derived information is available to help identify the setback distances required to effectively reduce the transport of contaminants in runoff. The objective of this study was to determine the effects of setback distance and runoff rate on the concentrations and mass transport rates of selected constituents following land application of beef cattle manure to a no-till cropland area. The study site had a residue cover of 8.84 Mg ha …


Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola Jan 2016

Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola

Department of Biological Systems Engineering: Papers and Publications

The need for accurate, real-time, reliable, and multi-scale soil water content (SWC) monitoring is critical for a multitude of scientific disciplines trying to understand and predict the Earth’s terrestrial energy, water, and nutrient cycles. One promising technique to help meet this demand is fixed and roving cosmic-ray neutron probes (CRNPs). However, the relationship between observed low-energy neutrons and SWC is affected by local soil and vegetation calibration parameters. This effect may be accounted for by a calibration equation based on local soil type and the amount of vegetation. However, determining the calibration parameters for this equation is labor- and time-intensive, …


A Multi-Sensor System For High Throughput Field Phenotyping In Soybean And Wheat Breeding, Geng Bai, Yufeng Ge, Waseem Hussain, P. Stephen Baenziger, George L. Graef Jan 2016

A Multi-Sensor System For High Throughput Field Phenotyping In Soybean And Wheat Breeding, Geng Bai, Yufeng Ge, Waseem Hussain, P. Stephen Baenziger, George L. Graef

Department of Biological Systems Engineering: Papers and Publications

Collecting plant phenotypic data with sufficient resolution (in both space and time) and accuracy represents a long standing challenge in plant science research, and has been a major limiting factor for the effective use of genomic data for crop improvement. This is particularly true in plant breeding where collecting large-scale field-based plant phenotypes can be very labor intensive and costly. In this paper we reported a multi-sensor system for high throughput phenotyping in plant breeding. The system comprised five sensor modules (ultrasonic distance sensors, thermal infrared radiometers, NDVI sensors, portable spectrometers, and RGB web cameras) to measure crop canopy traits …


Control Architecture For Multi-Robot System, John H. Posselius, Christopher A. Foster, Santosh K. Pitla, Scott A. Shearer, Joe D. Luck, Michael P. Sama, Rodrigo S. Zandonadi Jan 2016

Control Architecture For Multi-Robot System, John H. Posselius, Christopher A. Foster, Santosh K. Pitla, Scott A. Shearer, Joe D. Luck, Michael P. Sama, Rodrigo S. Zandonadi

Department of Biological Systems Engineering: Papers and Publications

A multiple robot control architecture including a plurality of robotic agricultural machines including a first and second robotic agricultural machine. Each robotic agricultural machine including at least one controller configured to implement a plurality of finite state machines within an individual robot control architecture (IRCA) and a global information module (GIM) communicatively coupled to the IRCA. The GIMs of the first and second robotic agricultural machines being configured to cooperate to cause said first robotic agricultural machine and said second agricultural machine to perform at least one agricultural task.


A Multi-Scale Comparison Of Modeled And Observed Seasonal Methane Emissions In Northern Wetlands, Xiyan Xu, William J. Riley, Charles D. Koven, Dave P. Billesbach, Rachel Y.-W. Chang, Roisin Commane, Eugenie S. Euskirchen, Sean Hartery, Yoshinobu Harazono, Hiroki Iwata, Kyle C. Mcdonald, Charles E. Miller, Walter C. Oechel, Benjamin Poulter, Naama Raz-Yaseef, Colm Sweeny, Margaret Torn, Steven C. Wofsy, Zhen Zhang, Donatella Zona Jan 2016

A Multi-Scale Comparison Of Modeled And Observed Seasonal Methane Emissions In Northern Wetlands, Xiyan Xu, William J. Riley, Charles D. Koven, Dave P. Billesbach, Rachel Y.-W. Chang, Roisin Commane, Eugenie S. Euskirchen, Sean Hartery, Yoshinobu Harazono, Hiroki Iwata, Kyle C. Mcdonald, Charles E. Miller, Walter C. Oechel, Benjamin Poulter, Naama Raz-Yaseef, Colm Sweeny, Margaret Torn, Steven C. Wofsy, Zhen Zhang, Donatella Zona

Department of Biological Systems Engineering: Papers and Publications

Wetlands are the largest global natural methane (CH4) source, and emissions between 50 and 70° N latitude contribute 10–30% to this source. Predictive capability of land models for northern wetland CH4 emissions is still low due to limited site measurements, strong spatial and temporal variability in emissions, and complex hydrological and biogeochemical dynamics. To explore this issue, we compare wetland CH4 emission predictions from the Community Land Model 4.5 (CLM4.5-BGC) with siteto regional-scale observations. A comparison of the CH4 fluxes with eddy flux data highlighted needed changes to the model’s estimate of aerenchyma area, which …


A Generalized Spatial Measure For Resilience Of Microbial Systems, Ryan S. Renslow, Stephen R. Lindemann, Hyun-Seob Song Jan 2016

A Generalized Spatial Measure For Resilience Of Microbial Systems, Ryan S. Renslow, Stephen R. Lindemann, Hyun-Seob Song

Department of Biological Systems Engineering: Papers and Publications

The emergent property of resilience is the ability of a system to return to an original state after a disturbance. Resilience may be used as an early warning system for significant or irreversible community transition; that is, a community with diminishing or low resilience may be close to catastrophic shift in function or an irreversible collapse. Typically, resilience is quantified using recovery time, which may be difficult or impossible to directly measure in microbial systems. A recent study in the literature showed that under certain conditions, a set of spatial-based metrics termed recovery length, can be correlated to recovery time, …


Switchgrass Storage Effects On The Recovery Of Carbohydrates After Liquid Hot Water Pretreatment And Enzymatic Hydrolysis, Noaa Frederick, Mengxing Li, Danielle Julie Carrier, Michael D. Buser, Mark R. Wilkins Jan 2016

Switchgrass Storage Effects On The Recovery Of Carbohydrates After Liquid Hot Water Pretreatment And Enzymatic Hydrolysis, Noaa Frederick, Mengxing Li, Danielle Julie Carrier, Michael D. Buser, Mark R. Wilkins

Department of Biological Systems Engineering: Papers and Publications

Perennial grasses that would be used for bioenergy and bioproducts production will need to be stored for various periods of time to ensure a continual feedstock supply to a bioprocessing facility. The effects of storage practices on grass composition and the response of grasses to subsequent bioprocesses such as pretreatment and enzymatic hydrolysis needs to be understood to develop the most efficient storage protocols. This study examined the effect of outdoor storage of round switchgrass bales on composition before and after liquid hot water pretreatment (LHW) and enzymatic hydrolysis. This study also examined the effect of washing LHW pretreated biomass …


Analyses, Calibration And Validation Of Evapotranspirationmodels To Predict Grass-Reference Evapotranspiration In Thesenegal River Delta, Koffi Djaman, Hossein Tabari, Alpha B. Balde, Lamine Diop, Koichi Futakuchi, Suat Irmak Jan 2016

Analyses, Calibration And Validation Of Evapotranspirationmodels To Predict Grass-Reference Evapotranspiration In Thesenegal River Delta, Koffi Djaman, Hossein Tabari, Alpha B. Balde, Lamine Diop, Koichi Futakuchi, Suat Irmak

Department of Biological Systems Engineering: Papers and Publications

Study region: Grass-reference evapotranspiration estimation by the Penman-Monteithmethod (PM-ETo) requires a number of climate variables which are not always availableat all weather stations. Different alternative ETo equations have been developed and theirutilization for various local climate conditions requires analyses of their accuracy as com-pared to the standardized Penman-Monteith method. There is a significant lack of data andinformation on this topic in the Senegal River Delta (SRD). Study focus: The objective of this study was to evaluate, calibrate and validate six EToequations ((Trabert, Mahringer, Penman1948, Albrecht, Valiantzas1 and Valiantzas2) forthe SRD. Although all six equations showed good agreement with the PM-ETo …


Protein-Targeted Corona Phase Molecular Recognition, Gili Bisker, Juyao Dong, Hoyoung D. Park, Nicole M. Iverson, Jiyoung Ahn, Justin T. Nelson, Markita P. Landry, Sebastian Kruss, Michael S. Strano Jan 2016

Protein-Targeted Corona Phase Molecular Recognition, Gili Bisker, Juyao Dong, Hoyoung D. Park, Nicole M. Iverson, Jiyoung Ahn, Justin T. Nelson, Markita P. Landry, Sebastian Kruss, Michael S. Strano

Department of Biological Systems Engineering: Papers and Publications

Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated …


Temporal Dynamics Of Maize Plant Growth, Water Use, And Leaf Water Content Using Automated High Throughput Rgb And Hyperspectral Imaging, Yufeng Ge, Geng Bai, Vincent Stoerger, James C. Schnable Jan 2016

Temporal Dynamics Of Maize Plant Growth, Water Use, And Leaf Water Content Using Automated High Throughput Rgb And Hyperspectral Imaging, Yufeng Ge, Geng Bai, Vincent Stoerger, James C. Schnable

Department of Biological Systems Engineering: Papers and Publications

Automated collection of large scale plant phenotype datasets using high throughput imaging systems has the potential to alleviate current bottlenecks in data-driven plant breeding and crop improvement. In this study, we demonstrate the characterization of temporal dynamics of plant growth and water use, and leaf water content of two maize genotypes under two different water treatments. RGB (Red Green Blue) images are processed to estimate projected plant area, which are correlated with destructively measured plant shoot fresh weight (FW), dry weight (DW) and leaf area. Estimated plant FW and DW, along with pot weights, are used to derive daily plant …


Analytical Solution For Speed To Achieve A Desired Operating Point For A Given Fan Or Pump, Michael F. Kocher, Jeyamkondan Subbiah Jan 2016

Analytical Solution For Speed To Achieve A Desired Operating Point For A Given Fan Or Pump, Michael F. Kocher, Jeyamkondan Subbiah

Department of Biological Systems Engineering: Papers and Publications

The Affinity Laws for fans (and pumps) provide a way of determining new fan or pump speed given fan or pump performance curve data and a desired operating point (combination of flow rate and pressure) that does not fall on the curve. However, the affinity law calculations require using a point on the curve (hereafter referred to as the “basic point”) to determine the new speed. Most references regarding the Affinity Laws do not give a clear description of the method for determining the “basic point”, and improper selection of this point can affect the results considerably. This article describes …


Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter Jan 2016

Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter

Department of Biological Systems Engineering: Papers and Publications

In this paper a fuzzy logic navigation controller for an inter-row agricultural robot is developed and evaluated in laboratory settings. The controller receives input from one-dimensional (1D) ranging sensors on the robotic platform, and operated on ten fuzzy rules for basic row-following behavior. The control system was implemented on basic hardware for proof of concept and operated on a commonly available microcontroller development platform and open source software libraries. The robot platform used for experimentation was a small tracked vehicle with differential steering control. Fuzzy inferencing and defuzzification, step response and cross track error were obtained from the test conducted …


Tractor Hydraulic Power Data Acquisition System, J. B.W. Roeber, Santosh Pitla, Michael F. Kocher, Joe D. Luck, Roger M. Hoy Jan 2016

Tractor Hydraulic Power Data Acquisition System, J. B.W. Roeber, Santosh Pitla, Michael F. Kocher, Joe D. Luck, Roger M. Hoy

Department of Biological Systems Engineering: Papers and Publications

Tractor hydraulic power is used on a wide range of agricultural implements; however, the availability of operational hydraulic data at points other than full engine throttle position is limited. Operators could utilize this hydraulic data to maximize field efficiency and minimize machinery costs when determining suitable machinery for field operations. A field usable hydraulic test apparatus capable of measuring tractor hydraulic pressure and flow rate data was developed. The goal of this study was to determine if a hydraulic flow and pressure measurement device could be installed on the rear of a tractor to provide implement hydraulic power consumption at …