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Full-Text Articles in Engineering
Effect Of Intermittent Applications Of Continuous Ultrasound Signal On The Morphology And Cytoskeletal Organization In Chondrocytes, Anuradha Subramanian, Sandra Noriega, Gulnara Hasanova
Effect Of Intermittent Applications Of Continuous Ultrasound Signal On The Morphology And Cytoskeletal Organization In Chondrocytes, Anuradha Subramanian, Sandra Noriega, Gulnara Hasanova
Anuradha Subramanian Publications
The cellular component of the articular cartilage, chondrocytes, have long been recognized as strain-sensitive cells, and have the ability to sense mechanical stimulation through surface receptors and intracellular signaling pathways. In the present study, a continuous ultrasound wave for predetermined time intervals was employed, as opposed to pulsed-ultrasound used in previous studies, to stimulate chondrocytes seeded in 3D scaffolds. Chondrocytes seeded in 3D scaffolds (n = 6) were subjected to stimulation by US as follows: 1.5 MHz for 161 s, 5.0 MHz for 51 s and 8.5 MHz for 24 s and the US signal was applied twice in a …
Control On Platelet Adhesion And Activation Events By Selective And Specific Binding Of Plasma Protein On Peptide-Coupled Surface, Anuradha Subramanian, Sanjukta Guha-Thankruta
Control On Platelet Adhesion And Activation Events By Selective And Specific Binding Of Plasma Protein On Peptide-Coupled Surface, Anuradha Subramanian, Sanjukta Guha-Thankruta
Anuradha Subramanian Publications
Implantable material surfaces suffer from unavoidable major events of blood–biomaterial interactions leading to thrombus formation and finally foreign material rejection. An albuminated surface can minimize the platelet adhesion and activation that reduces the chance of thrombus formation. The main objective of our work was to design surfaces having maximum human serum albumin (HSA) binding capacity over other plasma proteins in blood by covalently grafting custom made peptides having selectivity and specificity towards HSA. First, silicon wafer surfaces were chemically treated to generate –NH2 terminated self-assembled monolayer (SAM). SAMs were then covalently coupled with (a) peptides using 1- ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and …
Importance Of Annealing Times In The Pcr Amplification Of Gc-Rich Genes, Anuradha Subramanian, Tarlan Mamedov, Elsje Pinnear, Hendrik J. Viljoen
Importance Of Annealing Times In The Pcr Amplification Of Gc-Rich Genes, Anuradha Subramanian, Tarlan Mamedov, Elsje Pinnear, Hendrik J. Viljoen
Anuradha Subramanian Publications
This combined theoretical and experimental study conclusively demonstrates that annealing times play a crucial role in the PCR amplification of GC-rich templates. To assist with the DNA amplification of GC-rich targets, a theory has been developed that evaluates the fraction of annealed primer/polymerase complex at the correct binding site as a function of annealing temperature and annealing period. Optimum annealing times lie in the range of 3 to 6 s, and annealing times greater than 10 s yield smeared PCR amplified products. The theory has been applied to the PCR amplification of the following human cDNA templates: ARX gene (78.2%, …
Characterization Of A Polymer Surface With Sequentially Immobilized Proteins, Anuradha Subramanian, Tarlan Mammedov, Karl E. Kador
Characterization Of A Polymer Surface With Sequentially Immobilized Proteins, Anuradha Subramanian, Tarlan Mammedov, Karl E. Kador
Anuradha Subramanian Publications
To overcome the procoagulant processes on the surfaces of biomaterials, surface modifications have been undertaken to achieve hemocompatability characteristics that are comparable to the native endothelium. Our immediate goal in this paper is to design and develop strategies to inhibit thrombin activation on biomaterial surfaces. We will use biodurable polyurethane (PU) as the background polymer and synthesize biomaterial surfaces containing two immobilized recombinant proteins. To attain our objective, we have first undertaken the surface modification of biodurable polyurethane (chronoflex- AR) to enable the sequential immobilization of proteins via a bi-dentate bridge, a novel modification strategy. We have verified the creation …