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Full-Text Articles in Engineering
Genome-Scale Analysis Of Saccharomyces Cerevisiae Metabolism And Ethanol Production In Fed-Batch Culture, Radhakrishnan Mahadevan, Michael A. Henson
Genome-Scale Analysis Of Saccharomyces Cerevisiae Metabolism And Ethanol Production In Fed-Batch Culture, Radhakrishnan Mahadevan, Michael A. Henson
Michael A Henson
A dynamic flux balance model based on a genome-scale metabolic network reconstruction is developed for in silico analysis of Saccharomyces cerevisiae metabolism and ethanol production in fed-batch culture. Metabolic engineering strategies previously identified for their enhanced steady-state biomass and/or ethanol yields are evaluated for fed-batch performance in glucose and glucose/xylose media. Dynamic analysis is shown to provide a single quantitative measure of fed-batch ethanol productivity that explicitly handles the possible tradeoff between the biomass and ethanol yields. Productivity optimization conducted to rank achievable fed-batch performance demonstrates that the genetic manipulation strategy and the fed-batch operating policy should be considered simultaneously. …
A Molecular Model For Intercellular Synchronization In The Mammalian Circadian Clock, Tsz-Leung To, Michael A. Henson, Erik D. Herzog, Francis J. Doyle Iii
A Molecular Model For Intercellular Synchronization In The Mammalian Circadian Clock, Tsz-Leung To, Michael A. Henson, Erik D. Herzog, Francis J. Doyle Iii
Michael A Henson
The mechanisms and consequences of synchrony among heterogeneous oscillators are poorly understood in biological systems. We present a multicellular, molecular model of the mammalian circadian clock that incorporates recent data implicating the neurotransmitter vasoactive intestinal polypeptide (VIP) as the key synchronizing agent. The model postulates that synchrony arises amongcircadian neurons because they release VIP rhythmically on a daily basis and in response to ambient light. Two basic cell types, intrinsically rhythmic pacemakers and damped oscillators, are assumed to arise from a distribution of Period gene transcription rates. Postsynaptic neurons show time-of-day dependent responses to VIP binding through a signaling cascade …