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A Population Balance Equation Model Of Aggregation Dynamics In Taxus Suspension Cell Cultures, Martin E. Kolewe, Susan C. Roberts, Michael A. Henson
A Population Balance Equation Model Of Aggregation Dynamics In Taxus Suspension Cell Cultures, Martin E. Kolewe, Susan C. Roberts, Michael A. Henson
Michael A Henson
The nature of plant cells to grow as multicellular aggregates in suspension culture has profound effects on bioprocess performance. Recent advances in the measurement of plant cell aggregate size allow for routine process monitoring of this property. We have exploited this capability to develop a conceptual model to describe changes in the aggregate size distribution that are observed over the course of a Taxus cell suspension batch culture. We utilized the population balance equation framework to describe plant cell aggregates as a particulate system, accounting for the relevant phenomenological processes underlying aggregation, such as growth and breakage. We compared model …
Analysis Of Aggregate Size As A Process Variable Affecting Paclitaxel Accumulation In Taxus Suspension Cultures, Martin E. Kolewe, Michael A. Henson, Susan C. Roberts
Analysis Of Aggregate Size As A Process Variable Affecting Paclitaxel Accumulation In Taxus Suspension Cultures, Martin E. Kolewe, Michael A. Henson, Susan C. Roberts
Michael A Henson
Plant cell aggregates have long been implicated in affecting cellular metabolism in suspension culture, yet the rigorous characterization of aggregate size as a process variable and its effect on bioprocess performance has not been demonstrated. Aggregate fractionation and analysis of biomass-associated product is commonly used to assess the effect of aggregation, but we establish that this method is flawed under certain conditions and does not necessarily agree with comprehensive studies of total culture performance. Leveraging recent advances to routinely measure aggregate size distributions, we developed a simple method to manipulate aggregate size and evaluate its effect on the culture as …