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University of Massachusetts Amherst

Environmental Engineering

Civil and Environmental Engineering Faculty Publication Series

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In Vivo Evaluation Of Oxygenic Photogranules’ Photosynthetic Capacity By Pulse Amplitude Modulation And Phototrophic–Irradiance Curves, Joseph G. Gikonyo, Andrew Keyser, John Tobiason, Jeeyon Jeong, Chul Park Jan 2021

In Vivo Evaluation Of Oxygenic Photogranules’ Photosynthetic Capacity By Pulse Amplitude Modulation And Phototrophic–Irradiance Curves, Joseph G. Gikonyo, Andrew Keyser, John Tobiason, Jeeyon Jeong, Chul Park

Civil and Environmental Engineering Faculty Publication Series

The commingled microbial moiety of oxygenic photogranules (OPGs) facilitates aeration-free wastewater treatment. Embedded in an extracellular polymeric substances (EPS) matrix, microbial producers and consumers of oxygen occupying granular niches exchange substrates among themselves and with the bulk fluid. An assessment of the OPG's phototrophic potential or functional capacity may require combining different photoactivity signals. The photosynthetic capacity was evaluated using photosynthetic oxygen evolution (POE) and chlorophyll fluorescence (rapid light curves, RLC) measurements using OPGs grown at different light intensities.. A maximum oxygen generating capacity for optimal OPGs and reactor conditions was determined to be 284.4 mgO(2) gVSS(-1) h(-1) The OPGs …