Engineering Commons^™

Detection Of Escherichia Coli Using The Bacteriophage-Based Fluorescent Technique, Aung Khaing

Biological and Agricultural Engineering Undergraduate Honors Theses

The detection of viable Escherichia coli (E. coli) by the bacteriophage-based fluorescent technique using the enhanced green fluorescent protein (EGFP) was tested in this project. EGFP, a mutant of green fluorescent protein (GFP), was used due to its high fluorescent efficiency compared to wild-type GFP. First, E. coli bacteria were cultured to the mid-exponential phase in the 2× YT medium and then they were infected with the M13 bacteriophages containing the EGFP gene. Infected bacteria were incubated for different time intervals, and the expression of EGFP on the E. coli membrane was tested by the PerkinElmer LS 55 Luminescence Spectrometer, …