Engineering Commons^™

Expression, Production, And Purification Of Novel Therapeutic Proteins, Mckinzie Shea Fruchtl

Graduate Theses and Dissertations

Interest in the production of recombinant proteins consisting of collagen binding domain (CBD) fused to a bioactive material has increased due to the targeting/attachment capabilities of CBD. For example, CBD fusions can be applied to the reversing of bone density loss and the repair of the eardrum, specifically, by choosing an appropriate fusion partner (parathyroid hormone or epidermal growth factor). The production of CBD fusions was examined using batch and fed-batch culturing of Escherichia coli to express the fusion proteins, and affinity chromatography to isolate the final product.

Different medium formulations, feeding strategies, and induction methods were tested in order …

Structural Characterization Of Engineered Muscular Mimetic, Nadia Bhatti

Biological and Agricultural Engineering Undergraduate Honors Theses

An engineered muscular mimetic (EMM) was synthesized, and its overall structure—porosity, average pore size, and pore alignment—was characterized and compared to actual decellularized skeletal muscle (DSM). The EMM’s porosity was about 20% more and average pore size was about 85% smaller compared to that of the DSM. Unlike the strong horizontal pore alignment in the DSM, no distinct orientation could be identified in the EMM structure.