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Superoxide radical; Hydroxyl radical; Oxygen toxicity; Reperfusion; Reoxygenation; Reperfusion injury; Antioxidant; Iron chelator; Thiobarbituric acid; Malondialdehyde
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Full-Text Articles in Engineering
A Rapid, Widely Applicable Screen For Drugs That Suppress Free Radical Formation In Ischemia/Reperfusion, Steven C. Salaris, Charles F. Babbs
A Rapid, Widely Applicable Screen For Drugs That Suppress Free Radical Formation In Ischemia/Reperfusion, Steven C. Salaris, Charles F. Babbs
Weldon School of Biomedical Engineering Faculty Publications
Substantial injury can occur during reoxygenation of previously ischemic tissue in many experimental models, as the result of the generation of oxygen-derived free radicals. To test the antiradical activity of potentially protective compounds in this setting, we developed a simple screening system, applicable to fresh biopsy specimens, in which warm ischemia and reoxygenation of excised tissue are performed in vitro. Tissue production of malondialdehyde (MDA) equivalents is used as a nonspecific-but-sensitive marker of oxygen radical damage. Test compounds with putative antiradical activity are added prior to the reoxygenation phase, and their ability to suppress MDA production is an index of …
A Rapid, Widely Applicable Screen For Drugs That Suppress Free Radical Formation In Ischemia/Reperfusion, Steven C. Salaris, Charles F. Babbs
A Rapid, Widely Applicable Screen For Drugs That Suppress Free Radical Formation In Ischemia/Reperfusion, Steven C. Salaris, Charles F. Babbs
Weldon School of Biomedical Engineering Faculty Publications
Substantial injury can occur during reoxygenation of previously ischemic tissue in many experimental models, as the result of the generation of oxygen-derived free radicals. To test the antiradical activity of potentially protective compounds in this setting, we developed a simple screening system, applicable to fresh biopsy specimens, in which warm ischemia and reoxygenation of excised tissue are performed in vitro. Tissue production of malondialdehyde (MDA) equivalents is used as a nonspecific-but-sensitive marker of oxygen radical damage. Test compounds with putative antiradical activity are added prior to the reoxygenation phase, and their ability to suppress MDA production is an index of …