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Studies On Improved Integrated Membrane-Based Chromatographic Process For Bioseparation, Yanke Xu
Studies On Improved Integrated Membrane-Based Chromatographic Process For Bioseparation, Yanke Xu
Dissertations
To improve protein separation and purification directly from a fermentation broth, a novel membrane filtration-cum-chromatography device configuration having a relatively impermeable coated zone near the hollow fiber module outlet has been developed. The integrated membrane filtration-cum-chromatography unit packed with chromatographic beads on the shell side of the hollow fiber unit enjoys the advantages of both membrane filtration and chromatography; it allows one to load the chromatographic media directly from the fermentation broth or lysate and separate the adsorbed proteins through the subsequent elution step in a cyclic process.
Interfacial polymerization was carried out to coat the bottom section of the …
An Integrated Membrane-Based Chromatographic Process For Biomolecule Isolation And Purification, Xiaoping Dai
An Integrated Membrane-Based Chromatographic Process For Biomolecule Isolation And Purification, Xiaoping Dai
Dissertations
Biomolecules obtained from fermentation and cell-culture processes are present in low concentrations in a complex medium/broth. For intracellular products, cells are first harvested. Subsequently, cell lysis and homogenization are undertaken to produce a homogenate. The biomolecules are next separated from the cell debris by lysate clarification. For extracellular products, the biomolecules are separated from the whole cells by clarification. The overall yield is reduced with each additional step. As the cost of bioseparation process accounts for 50-80% of the final product cost, innovations in and improvement of bio-downstream processing are greatly needed.
In this research, a cyclic process integrating a …