Engineering Commons^™

Biodegradation Of Naphthalene Using Pseudomonas Putida (Atcc 17484) In Batch And Chemostat Reactors, Jay Boyd Best

Theses

Kinetic experiments were conducted using Pseudomonas putida (ATCC 17484) to determine the rate of growth when naphthalene was provided as a sole carbon source in suspended biomass systems. Batch and chemostat reactors were used under three sets of conditions: non-aerated, aerated at 29.5° C, and aerated at 25.5° C. A number of concentrations were tested under each set of conditions. Data regression was used to determine parameters for Monod (non-inhibitory), Andrews (inhibitory), and zero-order kinetics. Although the Andrews fit of the data provided a slightly lower sum-of-squares error (SSE), the amount of data scatter and the weak inhibition made the …

Integration Of Surfactants And Time Release Nutrients With Pneumatic Fracturing Process, Atiqur Md. Rahman

Theses

The objective of this laboratory study was the development of two novel improvements to the pneumatic fracturing process which would extend its present application. The first involved use of surfactant during pneumatic injection, and the second was subsurface injection of "time-release" dry nutrient pellets for enhancement of in situ biodegradation.

Bench scale tests demonstrated that pneumatic fracturing can be successfully performed with air containing a surfactant solution (foam fracturing). The results showed that foam fracturing followed by increased the rate of surrogate contaminant removal from 8% to 10% compared with regular pneumatic fracturing. These increases were attributed to enlarged fracture …

Integration Of Pneumatic Fracturing To Enhance In Situ Bioremediation, Conan Dante Fitzgerald

Theses

The purpose of this thesis was to study the anticipated benefits of integrating pneumatic fracturing with in situ bioremediation. Since pneumatic fracturing increases subsurface air flow in low permeability formations, it has the potential to overcome many of the major limiting factors of microbial growth and activity. A new innovation called pneumatic bio-injection can further enhance in situ bioremediation by efficiently dispersing biological solutions, including microorganisms, into a formation.

Bench scale experiments were conducted to examine the ability of microorganisms to survive the pressures and stresses associated with pneumatic injection. Tests conducted at pressures ranging from 60 to 500 psi …