Engineering Commons^™

3d Bioprinting Of Stem Cells And Polymer/Bioactive Glass Composite Scaffolds For Bone Tissue Engineering, Caroline Murphy, Krishna Kolan, Wenbin Li, Julie A. Semon, D. E. Day, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

A major limitation of using synthetic scaffolds in tissue engineering applications is insufficient angiogenesis in scaffold interior. Bioactive borate glasses have been shown to promote angiogenesis. There is a need to investigate the biofabrication of polymer composites by incorporating borate glass to increase the angiogenic capacity of the fabri-cated scaffolds. In this study, we investigated the bioprinting of human adipose stem cells (ASCs) with a polycaprolac-tone (PCL)/bioactive borate glass composite. Borate glass at the concentration of 10 to 50 weight %, was added to a mixture of PCL and organic solvent to make an extrudable paste. ASCs suspended in Matrigel …

3d Bioprinting A Pcl/13-93b3 Glass Composite And Its Potential Use As A Bio-Ink, Caroline Blair Murphy

Masters Theses

"A major limitation of using synthetic scaffolds in tissue engineering is little growth of incorporated cells in the interior of the scaffold, resulting in insufficient angiogenesis in the scaffold interior. Recently, cells have been 3D bioprinted concurrently with biomaterials to produce a cellularized, bioactive, angiogenic 3D environment. This thesis describes a novel solvent-extrusion method for printing polycaprolactone (PCL)/bioactive borate glass composite as a biomaterial for a cell-laden scaffold.

Bioactive borate glass was added to a mixture of PCL and organic solvent to make an extrudable paste, creating scaffolds measuring 10×10×1 mm³ in overall dimensions with pore sizes ranging from …