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Quantification Of Mitochondrial Membrane Potential In The Isloated Rat Lung Using R6g, Anthony Cammarata
Quantification Of Mitochondrial Membrane Potential In The Isloated Rat Lung Using R6g, Anthony Cammarata
Master's Theses (2009 -)
Mitochondrial membrane potential (Δm) plays a key role in vital mitochondrial functions, and its dissipation is a hallmark of mitochondrial dysfunction in various cell types. The objective of this study was to develop an experimental and computational approach for estimating Δm in intact rat lungs using the lipophilic fluorescent cationic dye rhodamine 6G (R6G). Rat lungs were isolated and connected to a ventilation-perfusion system. The experimental Protocol consisted of three single-pass phases: loading, wash, and uncoupling, in which the lungs were perfused with R6G-containing perfusate, fresh R6G-free perfusate, or R6G-free perfusate containing the mitochondrial uncoupler FCCP, respectively. This Protocol was …