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Single-Use Technologies: Bridging Polymer Science to Biotechnology Applications
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Cell Culture Scale Translation From A 24-Well Single-Use Miniature Bioreactor And Subsequent Impact On Product And Broth Quality, John Betts, Franc Baganz, Gary Lye, Steve Warr, Gary Finka, Mark Uden
Cell Culture Scale Translation From A 24-Well Single-Use Miniature Bioreactor And Subsequent Impact On Product And Broth Quality, John Betts, Franc Baganz, Gary Lye, Steve Warr, Gary Finka, Mark Uden
Single-Use Technologies: Bridging Polymer Science to Biotechnology Applications
To accelerate cell culture process development, most companies have validated scale-down models of their pilot and manufacturing scale bioreactors. Advancing such mimics to even smaller scales requires the large scale engineering environment to be accurately recreated. Here we describe a single-use microwell methodology that accurately reproduces not only cell growth kinetics but also key attributes related to product quality and broth processability.
The μ24 miniature bioreactor system enables system level control of agitation (by orbital shaking), with individual well control of pH, DO and temperature. Two distinct plate types are investigated, allowing for either headspace or direct gas sparging. An …
Challenges Of Scale Down Model For Disposable Bioreactors: Case Studies On Growth & Product Quality Impacts, Jincai Li, Guangming Zhang, Huilin Zhu
Challenges Of Scale Down Model For Disposable Bioreactors: Case Studies On Growth & Product Quality Impacts, Jincai Li, Guangming Zhang, Huilin Zhu
Single-Use Technologies: Bridging Polymer Science to Biotechnology Applications
Despite wide-spread use of disposable bioreactors, there is a lack of well-established scale-down model for larger scale SUBs. Here we report a case of NS0 cell culture process transfer from 2000L stainless steel bioreactor (SST) to 2000L disposable bioreactor (SUB). Initial attempts in trying to grow the NS0 cells in the small scale 2D bags yielded non-satisfactory results, as growth was impacted by bag material type as well as by suppliers of the same bag material type. However, 3D bags of 50L and above proved to be supportive of the NS0 cell line growth.
Even for cell lines that do …