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Engineering Conferences International
Single-Use Technologies: Bridging Polymer Science to Biotechnology Applications
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Cell Culture Scale Translation From A 24-Well Single-Use Miniature Bioreactor And Subsequent Impact On Product And Broth Quality, John Betts, Franc Baganz, Gary Lye, Steve Warr, Gary Finka, Mark Uden
Cell Culture Scale Translation From A 24-Well Single-Use Miniature Bioreactor And Subsequent Impact On Product And Broth Quality, John Betts, Franc Baganz, Gary Lye, Steve Warr, Gary Finka, Mark Uden
Single-Use Technologies: Bridging Polymer Science to Biotechnology Applications
To accelerate cell culture process development, most companies have validated scale-down models of their pilot and manufacturing scale bioreactors. Advancing such mimics to even smaller scales requires the large scale engineering environment to be accurately recreated. Here we describe a single-use microwell methodology that accurately reproduces not only cell growth kinetics but also key attributes related to product quality and broth processability.
The μ24 miniature bioreactor system enables system level control of agitation (by orbital shaking), with individual well control of pH, DO and temperature. Two distinct plate types are investigated, allowing for either headspace or direct gas sparging. An …
Engineering Characterisation Of A Rocked-Bag Bioreactor To Evaluate Key Effectors Of Successful Mammalian Cell Culture, Douglas Marsh, Matthew Osborne, Martina Micheletti, Gary Lye
Engineering Characterisation Of A Rocked-Bag Bioreactor To Evaluate Key Effectors Of Successful Mammalian Cell Culture, Douglas Marsh, Matthew Osborne, Martina Micheletti, Gary Lye
Single-Use Technologies: Bridging Polymer Science to Biotechnology Applications
Engineering characterisation is essential for efficient and knowledge-led process development in biomanufacturing and to underpin the adoption of single-use technologies [1]. Despite diverse applications of rocked bag bioreactors, there is currently little understanding of the fundamental determinants of fluid mixing and mass transfer, which are important for mammalian cell culture.
In this work, a flexible single-use rocked bag bioreactor system has been fully evaluated in terms of volumetric oxygen mass transfer coefficient (kLa), CO2 stripping rate and liquid phase mixing time (tm) at 10, 20 and 50L scale. Five inputs were identified as potentially affecting …