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The Microbial Antibodies Secretion Expression Platform With Scale Down Fermentors, Jen-Wei Chang, Wei-Kuang Chi, Neng-Hsien Chang, Wei-Hong Chen, Yi-Hua Huang, Chih-Hsi Fan

Biochemical and Molecular Engineering XX

Therapeutic antibodies have become one of the most effective therapeutics for human diseases such as cancer, inflammation and viral infection. The production of antibody-based drugs using microbial expression systems is more cost effective with ease of gene manipulation compared to mammalian expression systems. In our team, antibody fragments (ex: BsAb, scFv and Fab) were produced from methylotrophic yeast Pichia pastoris secretion expression system with the AOX1 as driven promoter or E. coli secretion expression system. To achieve high production yield for both system, we investigated fermentation parameter such as base medium, induction medium, induction condition, feeding strategy and pH. For …